根皮苷对LO2正常人肝细胞体外细胞毒性的实验研究  

Cytocoxicity of Phloridzin on LO2 human liver cells

作　　者：陈署贤[1] 郭建茹[2] 朱冬梅[3] 刘华桢[3] 林继宗[1] 侯少贞[3] 

机构地区：[1]中山大学附属第三医院肝病外科,广州510630 [2]澳门科技大学中药质量研究国家重点实验室,澳门999078 [3]广州中医药大学中药学院,广州510006

出　　处：《吉林中医药》2015年第11期1159-1161,共3页Jilin Journal of Chinese Medicine

基　　金：国家自然科学基金(81303281);广东省教育部产学研结合项目(2012B091100184);广州中医药大学优秀青年学者科研基金项目(2012)

摘　　要：目的探究根皮苷对正常人肝细胞LO2的体外细胞毒性。方法实验分为正常组和根皮苷组。将从甜茶中提取根皮苷配以不同浓度根皮苷的培养液,浓度分别为4.0、8.0、16.0、32.0、64.0、128、256、512、800、1 000μmol/L。分别在24、48和72 h,使用MTT比色法测定并计算出细胞活力率。结果给药24、48和72 h后,根皮苷在4.0∽1 000μmol/L浓度的范围内对正常人肝细胞LO2无毒性作用,细胞的存活率在90%以上。结论在4.0∽1 000μmol/L浓度范围内,根皮苷对正常人肝细胞的正常增殖无明显抑制,未显示有肝细胞毒性作用。Objectlve To explore the cytotoxicity of Phloridzin on LO2 human liver cells. Methods The normal group and Phlorizin groups with the concentrations of 4.0,8.0,16.0,32.0,64.0,128,256,512,800,1 000μmol/L were established. Then the rate of cell viability was calculated by the MTF assay,respectively at 24 h ,48 h and 72 h after administration. Results After administration for 24,48 and 72 hours, the absorbance of all Phlorizin groups were similar to that of normal group, and there were no statistical significance. The cell viability was all above 90%. Con- clusion Phlorizin has no toxicity to normal human liver cells with the concentration range of 4.0- 1 000 μmol/L

关 键 词：根皮苷 肝细胞 MTT 

分 类 号：R285.5[医药卫生—中药学]