内皮素-1和肿瘤坏死因子-α在急性低压缺氧大鼠肾组织中的表达和意义  

Expression and significance of endothelin-1 and tumor necrosis factor-α in renal tissue of rats with acute hypobaric hypoxia

作　　者：牛天慧[1] 郭广进[1] 陆承荣[1] 罗渊[1] 王喆[1] 

机构地区：[1]空军总医院临床航空医学中心实验室,北京100142

出　　处：《中国医药导报》2015年第34期23-26,共4页China Medical Herald

基　　金：空军总医院面上项目(kz2012041)

摘　　要：目的观察内皮素-1(ET-1)和肿瘤坏死因子-α(TNF-α)在急性低压缺氧大鼠肾组织中的表达,探讨低压缺氧条件下肾损伤的机制。方法将16只雄性Wistar大鼠,随机分为地面组和8.0 km缺氧组,每组各8只。用完全随机法,采用实验对照方式,进行纵向观察研究。将动物置于小型动物低压舱内,以30 m/s的速度模拟上升至8.0 km高度,暴露30 min,造成缺氧,然后以30 m/s的速度下降至地面;地面组同时放入另一实验条件完全相同的动物低压舱30 min,不进行低压缺氧。随后采集标本,用免疫组织化学方法检测肾脏中ET-1和TNF-α的表达情况;用酶联免疫吸附(ELISA)方法测量血清中超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性和丙二醛(MDA)含量的水平。结果急性低压缺氧后经免疫组织化学检测,8.0 km组与地面组比较,ET-1和TNF-α阳性表达均明显增强,与地面组比较阳性表达率显著增高,差异有统计学意义(P<0.05)。8.0 km组大鼠血清中,SOD和CAT活性不变(P﹥0.05),MDA含量明显增高,差异有统计学意义(P<0.05)。结论急性低压缺氧发生肾损伤的病理改变与ET-1和TNF-α阳性表达率显著增高有明显的相关性。Objective To observe the expression of endothelin-1（ET-1） and tumor necrosis factor-α（TNF-α） in renal tissue of rats with acute hypobaric hypoxia and the mechanism of renal injury under hypobaric hypoxia conditions.Methods 16 male Wistar rats were randomly divided into ground group and 8.0 km group, Completely randomized controlled trial was conducted between experimental group and control group. 8 rats for each group. 8.0 km group animals were placed in micro-hypobaric chamber where the pressure was simulated up to 8.0 km altitude at the rising speed 30m/s. After 30 minutes of hypoxia, the rats were gradually recovered to the ground pressure at the speed of 30 m/s. All specimens were collected after experiment. Subsequently, immunohistochemical method was used to detect the expression levels of ET-1 and TNF-α in kidney and enzyme linked immunosorbent assay（ELISA） method was utilized to measure the content of malondialdehyde（MDA） and the activities of superoxide dismutase（SOD）, catalase（CAT） in serum. Results Compared with the ground group, the expression levels of ET-1 and TNF-α were increased remarkably（P〈0.05） in 8.0 km group, and the content of MDA in 8.0 km group was enhanced significantly（P〈0.05）. Nevertheless, SOD and CAT activities were not significantly changed. Conclusion Pathological changes in kidney under acute hypobaric hypoxia conditions are significantly correlated with the expression level of ET-1 and TNF-α.

关 键 词：急性低压缺氧 大鼠 内皮素-1 肿瘤坏死因子Α 

分 类 号：R692.5[医药卫生—泌尿科学]