ApoE基因敲除高脂血症小鼠下颌下腺超微结构及上游刺激因子1表达观察  

Observation of submandibular gland ultrastructure and upstream stimulatory factor 1 expression in hyperlipidemic Apo E knockout mice

作　　者：李静[1,2] 黄大可[3] 桂丽[3] 贾雪梅[1] 

机构地区：[1]安徽医科大学组织学与胚胎学教研室,合肥230032 [2]安徽医学高等专科学校图文信息中心,合肥230601 [3]安徽医科大学综合实验室,合肥230032

出　　处：《安徽医科大学学报》2015年第12期1725-1728,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学基金研究项目(编号:KJ2012A164)

摘　　要：目的观察载脂蛋白E(Apo E)基因敲除高脂血症(HLP)对小鼠下颌下腺超微结构以及上游刺激因子1(USF1)表达影响。方法 10只野生型小鼠予普通饲料喂养作为对照组,10只Apo E基因敲除子鼠予高脂饲料喂养作为高脂组。造模6个月后,取眼眶血检测血脂;取小鼠下颌下腺组织分别进行光镜、电镜和免疫组织化学观察。结果与对照组比较,高脂组胆固醇、三酰甘油和极低密度脂蛋白含量明显升高(P<0.05)。HE染色观察到,高脂组小鼠下颌下腺的腺泡明显萎缩,细胞排列紊乱。电镜下,可见高脂组小鼠导管上皮内线粒体嵴断裂,腺细胞内粗面内质网囊状扩张,结构松散零乱。免疫组化显示,高脂组USF1表达下降;平均光密度值与对照组比较差异有统计学意义(P<0.05)。结论 HLP可导致小鼠下颌下腺超微结构出现病理改变,USF1表达减少。Objective To observe the expression of apolipoprotein E （ApoE） gene knocking out hyperlipidemia （HLP） in mice submandibular gland uhrastructure and upstream stimulating factor 1 （ USF1 ）. Methods Take 10 wild mice fed with normal feed as control group, 10 ApoE knockout mice fed with high fat as hyperlipidemia group. Six months after the model was set up, blood fat in orbital blood was tested. The submandibular gland tissues of the mice were observed by using light microscopy, electron microscopy and immunohistoehemistry staining. Results Compared with the control group, the cholesterol, triglycerides and very low - density lipoprotein of high - fat group were significantly higher （P 〈 0. 05 ）. HE staining was observed, the acini of the submandibular glands in hyper- lipidemia group was atrophied obviously, and the cells were in disorder. Under electron microscopy, the subman- dibular gland cell mitochondria crests of mice in hyperlipidemia group were fractured, and rough endoplasmic retic- ulum eystically expanded, which was loose and messy. Immunohistochemical results showed that the USF1 expres- sion in hyperlipidemia group decreased; the difference in average optical density value and the control group was statistically significant （P 〈 0. 05）. Conclusion HLP can result in pathological changes in the mouse submandib- ular gland ultrastructure and reduction in USF1 expression.

关 键 词：高脂血症 超微结构 上游刺激因子1 下颌下腺 小鼠 

分 类 号：R332[医药卫生—人体生理学]