暗纹东方鲀渗透压调节机制的研究进展  

Research progress in osmoregulatory mechanism of Takifugu fasciatus

作　　者：王丽[1] 王小鲁[1] 梁奋飞[1] 尹绍武[1] 钱晓明[2] 卢玉平[2] 

机构地区：[1]南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,南京210023 [2]江苏中洋集团股份有限公司,南通226600

出　　处：《海洋渔业》2015年第6期575-583,共9页Marine Fisheries

基　　金：江苏省科技支撑计划(农业项目)(BE2013345);江苏省科技支撑计划(农业项目)(BE2013345)

摘　　要：渗透压调节与鱼类生命活动密切相关。近年来,广盐性鱼类复杂的渗透压调节机制逐渐成为国内外鱼类生理学研究热点之一。然而,国际上对硬骨鱼类盐度调控的相关研究更多的偏重于渗透压调节机理,研究层次虽广,但深度不够。暗纹东方鲀(Takifugu fasciatus)作为典型的广盐性鱼类,也是我国重要的养殖鱼类之一。本文从渗透压调节分子(离子转运体如Na^+,K^+-ATPase)、细胞(氯细胞)、组织器官(鳃、肾、肠)、系统的水平上总结了近年来有关暗纹东方鲀渗透压调节方面的研究成果,并在此基础上对下一步的重点研究方向进行了展望。本综述可为全面了解暗纹东方鲀渗透压调节的研究进展及对广盐性鱼类渗透压调节机制的研究提供参考。Osmoregulation has a close relationship with life activities of fish. Recently, the research of complicative osmoregulatory mechanism in euryhaline fish has been a hotspot at home and abroad. However, the study on salinity regulation in bony fishes lay more particular stress on osmoregulatory mechanism, the level is broad but not deep enough. As a typical euryhaline fish, Takifugu fasciatus is also an important economic fish in China. This study summarized the recent relevant achievements in T. fasciatus from the following aspects： （1） Osmoregulatory tissues and organs： T. fasciatus has a strong ability of osmotic adjustment and forms a higher osmoregulatory mechanism, osmoregulation is completed via ions and water co- transport by gills, kidney and intestine; （2） Hormonal level： based on real-time fluorescence quantitative and immunohistochemical method, T. fasciatus,adrenal medullary AM1 expresses in osmoregulatory organs of gill and kidney, and plays an important role in regulating the glomerular filtration and renal tubular reabsorption; （3） Cellular level： the immune cells in chemical research and transmission electron microscope shows that chlorine cells with ion transport ability highly cells can show a series of adjustments on changes, which can cause the gill＇ s changes enrich in the gill＇ s filament epithelium of T. fasciatus. These size, quantity and structure complexity while the enviroment of ion transport way and ability ; （4） Protein level： based on situ hybridization and immunohistochemical method, ion channel proteins in osmoregulation of T. fasciatus includes Na＋ , K＋ ATPase, HCO3 transporter （ NBCel / Slc26a6A and Slc26a6B）, SO42 transporter （SIc26a6A） and so on, and they each show different expression features for salinity changes; （5）Genetic level： based on real-time fluorescence quantitative methods, T. fasciatus changes the expression level of ion transport genes NKA, NKCC, NCC, AQP1, AQP3 and hormonal gene PLR to adapt to physiological react

关 键 词：暗纹东方鲀 离子转运体 Na+ K+-ATPase 广盐性 渗透压调节 

分 类 号：S917[农业科学—水产科学]