2型糖尿病足病患者血清与足部肌肉组织中SPARC水平的变化  

Variations of SPARC levels in serum and foot muscle tissue of patients with type 2 diabetic foot

作　　者：安盟盟 宋海燕[1] 刘浩[2] 朱莉莉[1] 王政[1] 杨鲁杰 

机构地区：[1]哈尔滨医科大学附属第二医院内分泌代谢病科,150086 [2]哈尔滨医科大学附属第二医院急诊创伤外科,150086

出　　处：《中华内分泌代谢杂志》2015年第11期961-966,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金（81370903）;教育部留学归国人员基金（20071108）;中国博士后基金（20060390237）;黑龙江省留学归国人员基金（LC06C23）

摘　　要：目的 观察富含半胱氨酸的酸性分泌蛋白（secreted protein acidic and rich in cysteine,SPARC）在2型糖尿病足病患者血清及足部肌肉组织中表达水平的变化.方法 选取2型糖尿病未合并下肢动脉血管、周围神经病变患者40例作为DMA组,2型糖尿病合并下肢动脉血管、周围神经病变患者40例作为DMB组,2型糖尿病足病患者40例作为DF组,以及健康体检者（包括血脂异常）40名作为NC组;DF组及NC组各6例取肌肉组织.分别采取ELISA法检测血清SPARC水平,RT-PCR、Western印迹法、免疫荧光法、免疫组化法检测肌肉组织SPARC mRNA和蛋白的表达水平.结果 DMA组及DMB组血清SPARC水平高于NC组及DF组[（1 040.48±212.12、1 068.36±165.45对841.93±144.57、835.43±188.37）ng/L,P＜0.01],DF组与NC组、DMA组与DMB组之间无差异统计学意义（P＞0.05）.DF患者足部肌肉组织中SPARC mRNA和蛋白呈高表达（P＜0.01）.结论 DF患者足部肌肉组织中SPARC mRNA和蛋白表达增高,表明SPARC可能参与糖尿病足损伤肌肉组织的修复及愈合.Objective To observe secreted protein acidic and rich in cysteine （SPARC） levels in serum and foot muscle tissue of patients with type 2 diabetic foot （DF）.Methods All participants were divided into four groups with 40 cases in each group, including type 2 diabetic patients without （DMA） and with （DMB） lower limb arterial sclerosis and peripheral neuropathy, DF group, and normal control （NC） group.The muscle tissues of foot from DF group （n =6） and NC group （n =6） were taken.Serum SPARC level was measured with ELISA.RT-PCR, Western blot, immunofluorescence, and immunohistochemistry were used to examine SPARC mRNA and protein expressions in the foot muscle.Results Serum SPARC levels were higher in DMA and DMB groups compared with NC and DF groups[（1 040.48 ±212.12 and 1 068.36 ± 165.45 vs 841.93 ± 144.57 and 835.43 ± 188.37） ng/L, P〈 0.01].There was no significant difference in serum SPARC level between DF and NC groups or DMA and DMB groups （P〉0.05）.The expression levels of SPARC mRNA and protein in the foot muscle were higher in DF compared with NC group （P〈0.05）.Conclusion SPARC mRNA and protein expressions in foot muscle tissue are higher in DF group compared with control group, indicating that SPARC may participate in the repair and healing of damaged muscle tissue of diabetic foot.

关 键 词：富含半胱氨酸的酸性分泌蛋白 糖尿病 2型 糖尿病足 DIABETES mellitus type 2 

分 类 号：R587.2[医药卫生—内分泌]