补脏通络方对糖尿病合并大血管病变大鼠主动脉内膜MMP-9/TIMP-1的调节  

The regulation of BuZangTongLuo Decoction on MMP-9/TIMP-1 in aortic tunica intima of diabetic rats with vascular disease

作　　者：杨化冰[1] 张翀[1] 吴珂[2] 秦保和 

机构地区：[1]湖北中医药大学基础医学院,湖北武汉430065 [2]武汉大学医学院基础医学院,湖北武汉430071 [3]湖北中医药大学黄家湖医院,湖北武汉430065

出　　处：《时珍国医国药》2015年第11期2577-2579,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金青年基金(No.81202733);湖北省武汉市科技局科技攻关计划(武科计[2013]20号)

摘　　要：目的观察补脏通络方对糖尿病合并大血管病变大鼠主动脉内膜MMP-9/TIMP-1表达的影响,探讨其逆转糖尿病大血管内膜增厚的可能机制。方法脂肪乳灌胃21d后一次性尾静脉注射STZ 30mg/kg造模。补脏通络方治疗。病理切片光镜观察大鼠胸主动脉内膜形态改变,检测内膜厚度;免疫组化检测主动脉内膜IV型胶原表达;免疫印迹法分析主动脉内膜MMP-9、TIMP-1蛋白表达。结果与正常组相比,病理组主动脉内膜增厚明显(P<0.01),IV型胶原表达增加(P<0.01),MMP-9、TIMP-1表达增加(P<0.01),MMP-9/TIMP-1比值上升(P<0.01);经补脏通络方治疗,与病理组相比,各治疗组主动脉内膜厚度和IV型胶原表达降低(P<0.01),MMP-9表达降低(P<0.01),TIMP-1表达增加(P<0.01),MMP-9/TIMP-1比值下降(P<0.01);补脏通络方高剂量组降低MMP-9、升高TIMP-1的作用优于低剂量组(P<0.05)。结论调节大血管内膜MMP-9/TIMP-1平衡,加速胶原降解,可能是补脏通络方逆转糖尿病大血管内膜增厚的可能机制。Objective To study the effect of BuZangTongLuo Decoction on the expression of MMP - 9/TIMP - 1 in aortic tunica intima of diabetic rats with vascular disease and discuss the mechanism of BuZangTongLuo Decoction intervening diabetic large vascular disease. Methods Rats fed with fat milk and injected with STZ30mg/kg to make mold and treated with BuZangTongLuo Decoction. To observe the pathological sections of aortic tunica intima with Light microscopic and the expression of collagen type IV, MMP -9,TIMP - 1 with Immunohistochemistry and Western blot. Results Compared with normal group, the aortic tunica inti- ma of pathological group was thicken significantly （ P 〈 0. 01 ） and the expression of collagen type IV, MMP - 9 ,TIMP - 1 were in- creased （P 〈 0.01 ） with MMP -9/TIMP - 1 increased（ P 〈 0.01 ）. After treated with BuZangTongLuo Decoction, compared with pathological group, the expression of collagen type IV, MMP - 9 in aortic tunica intima were reduced （P 〈 0.01 ） with TIMP - 1 increased （ P 〈 0.01 ） and MMP - 9/TIMP - 1 decreased（ P 〈 0. 01 ）. Conclusion The mechanism of BuZangTongLuo Decoction intervening diabetic large vascular disease may be relate to it＇s regulation on MMP - 9/TIMP - 1 in aortic tunica intima.

关 键 词：2型糖尿病 基质金属蛋白酶9(MMP-9) 金属蛋白酶抑制剂1(TIMP-1) 补脏通络方 

分 类 号：R285.5[医药卫生—中药学]