BRMS1基因在人鼻咽癌细胞株中表达水平的研究  

Expression levels of BRMS1 gene in human nasopharyngeal cell lines

作　　者：孙谦[1] 江浩[1] 汪庚明[1] 徐洪波[1] 张亚军[1] 张雷[1] 周育夫[1] 

机构地区：[1]安徽省蚌埠医学院第一附属医院肿瘤放疗科,233004

出　　处：《中华全科医学》2015年第12期1938-1940,F0003,共4页Chinese Journal of General Practice

基　　金：蚌埠医学院课题(Byky1240)

摘　　要：目的在mRNA水平检测5株不同转移习性的人鼻咽癌细胞株中BRMS1基因表达的差别,为进一步证实BRMS1基因是否在人鼻咽癌细胞株中有抑制转移作用且抑制转移能力是否与该5种细胞株转移习性具有一致性,为课题组进行下一步组织学实验以及动物实验提供细胞理论基础。方法体外培养5株人鼻咽癌细胞株CNE-1、SUNE-1、SUNE-1-5-8F、CNE-2Z、SUNE-1-6-10B,分别提取该5株细胞株中mRNA,应用实时荧光定量PCR法检测该5株细胞株中BRMS1基因mRNA的表达水平是否存在差异。结果 BRMS1基因的mRNA在5株细胞株中均有表达,但表达水平存在差异。SUNE-1-6-10B表达量为标准值,SUNE-1-5-8F、SUNE-1、CNE-2Z、CNE-1中BRMS1 mRNA的相对表达量与标准值SUNE-1-6-10B表达量相比为标准值的(23±1)%、(49±2)%、(81±1)%、(86±2)%,较SUNE-1-6-10B细胞株中BRMS1 mRNA表达水平分别下降了77%、51%、19%、14%(P<0.05)。结论 BRMS1基因在5株不同转移习性人鼻咽癌细胞株中mRNA的表达水平具有差异,在高转移习性细胞株中表达量低,而在低转移习性细胞株中表达量高,且其mRNA表达量随着细胞株的转移能力的增加而下降,从以上结果说明在鼻咽癌转移过程中BRMS1基因可能起到抑制作用。Objective To detect the expression levels of BRMS1 mRNA in five cell lines of nasopharyngeal carcinoma with different metastasis potentials,evaluate the inhibition of BRMS1 gene on metastasis and the relevance with the metastasis potentials of cells,and provide a theoretical basis for the next step histological cell experiments and animal experiments. Methods The nasopharyngeal carcinoma cell lines CNE-1,SUNE-1,SUNE-1-5-8F,CNE-2Z and SUNE-1-6-10 B were in vitro cultured. The expressions of mRNA in the five lines were detected by using real-time quantitative PCR. The results were compared. Results BRMS1 gene mRNA expressed in all the five cell lines and the expression level was different. SUNE-1-6-10 B was at the standard value; Compared with SUNE-1-6-10 B,SUNE-1-5-8F,SUNE-1,CNE-2Z,CNE-1and BRMS1 mRNA was（ 23 ± 1） %,（ 49 ± 2） %,（ 81 ± 1） % and（ 86 ± 2） % of the standard value,there was all positive expression of BRMS1 mRNA,the expression levels decreased obviously（ P〈0. 05）. Conclusion The BRMS1 mRNA expressed in CNE-1,SUNE-1,SUNE-1-5-8F,CNE-2Z and SUNE-1-6-10 B at different levels,and negatively correlated with the metastasis potentials of cell lines. The decreased expression levels of BRMS1 mRNA in the higher metastasis potentials lines reveals that BRMS1 gene may play an inhibitory effect in nasopharyngeal carcinoma metastasis.

关 键 词：鼻咽肿瘤 转移抑制基因 BRMS1 实时荧光定量PCR 

分 类 号：R139.6[医药卫生—劳动卫生] R446.7[医药卫生—公共卫生与预防医学]