时间分辨荧光免疫法定量检测乙肝病毒血清标志物的临床意义  

Clinical Effect of Time-resolved Fluorescence Immunoassay in Quantitative Detection of HBV M

作　　者：马春静 邹薇[2] 王丽 

机构地区：[1]齐齐哈尔建华医院检验科,黑龙江齐齐哈尔161000 [2]齐齐哈尔医学院第二附属医院输血科,黑龙江齐齐哈尔161000 [3]齐齐哈尔市中医医院检验科,黑龙江齐齐哈尔161000

出　　处：《中外医疗》2015年第17期188-189,共2页China & Foreign Medical Treatment

摘　　要：目的对时间分辨荧光免疫法定量检测乙肝病毒血清标志物的临床意义进行观察分析。方法整群选取2012年8月—2014年8月于该院进行救治的188例乙肝病毒患者,用酶联免疫吸附试验(ELISA)和时间分辨荧光免疫分析对患者的血清标本进行乙肝标志物的定性、定量检测和比较,并且探讨其结果。结果在HBsAg和HBeAg的检出的敏感度上,两种方法差异无统计学意义(P>0.05),TRFIA方法在抗-HBs、抗-HBe,抗-HBc的检出的敏感度明显比ELIASA法高,差异有统计学意义(P<0.05);两种方法在1,3,5(+)、1,5(+)、1,4,5(+)及其他模式中检出率差异无统计学意义(P>0.05);TRFIA在2(+)和2,4,5(+)检测阳性率比ELSIA差异有统计学意义(P<0.05),全阴性模式中TRFIA阳性率显著低于ELSIA(P<0.05)。结论 TRFIA法测定乙肝病毒血清标志物的相对灵敏度高,而且能够定量,值得临床推广应用。Objective To investigate the application of the time-resolved fluorescence immunoassay in the quantitative detection of HBV M（hepatitis b virus serological markers）.Methods 188 in-patients with hepatitis treated in our hospital from August 2012 to August 2014 were selected.Two kinds of methods,TRFIA（time-resolved fluorescence immunoassay） and ELISA（enzyme-linked immunosorbent assay） were used for the qualitative and quantitative detections and comparison of HBV M,and the results were discussed.Results There was no remarkable significance between ELISA and TRFIA in detecting HBsAg and HBeAg（P〈0.05）;but TRFIA method for the determinations of anti-HBs、anti-HBe and anti-HBc had high sensitivity（P〈0.05）;no obvious differences were found between ELISA and TRFIA in detecting 1,3,5（＋） and 1,5（＋） and 1,4,5（＋）and other modes（P〈0.05）;the positive detecting rate of TRFIA in 2（＋） and 2,4,5（＋） modes was better（P〈0.05）;the positive detecting rate of TRFIA in all negative mode was less（P〈0.05）.Conclusion Time-resolved fluorescence immunoassay for detection of HBV M has good sensitivity and good specificity and it is helpful to estimate the effect.

关 键 词：时间分辨荧光免疫法 定量 检测 乙肝病毒 血清标志物 

分 类 号：R446[医药卫生—诊断学]