Effect of porous titanium coated with IGF-1 and TGF-β_1 loaded gelatin microsphere on function of MG63 cells  

载IGF-1与TGF-β_1明胶微球涂层的多孔钛对MG63细胞功能的影响（英文）

作　　者：陈良建[1,2] 陈畅[1] 乔雪岩 余琨[2,3] 谢丽子[1] 曹君[1] 刘蓓蕾[1] 颜阳[3] 

机构地区：[1]中南大学湘雅三医院,长沙410013 [2]中南大学粉末冶金国家重点实验室,长沙410083 [3]中南大学材料科学与工程学院,长沙410083

出　　处：《Transactions of Nonferrous Metals Society of China》2015年第9期2974-2985,共12页中国有色金属学报（英文版）

基　　金：Project(2013zzts306)supported by the Fundamental Research Funds for the Central Universities of Central South University,China;Project(225)supported by the High Level Health Personnel in Hunan Province,China

摘　　要：Porous titanium with porosity of 60% was prepared by metal injection molding（MIM）,and coated with gelatin sustained-release microspheres which were made by improved emulsified cold condensation method.The effects of porous titanium coated with insulin-like growth factor-1（IGF-1） and transforming growth factor-β1（TGF-β1） gelatin microspheres on the function of MG63 cells were evaluated in vitro.The results show that porous titanium coated with gelatin sustained-release microspheres has no cytotoxicity.The IGF-1 and TGF-β1 loading concentrations are positively correlative with the proliferation and differentiation of MG63 after co-culturing with the concentrations of IGF-1 and TGF-β1 gelatin microspheres in the range of 0.1-10 ng/mg and 0.25-2.5 ng/mg,respectively.The MG63 cells exhibit the best proliferation and differentiation with the IGF-1 and TGF-β1 loading concentrations of 10 ng/mg and 2.5 ng/mg,respectively.The joint application of IGF-1 and TGF-β1 group,which promote adhesion,proliferation and differentiation of MG63 cells,is superior to a single application group.用金属注射成形（MIM）技术制备孔隙度为60%的多孔钛,用改良冷凝聚合交联法制备明胶缓释微球并涂覆于多孔钛表面,体外细胞评价胰岛素生长因子-1（IGF-1）、转化生长因子-β1（TGF-β1）明胶缓释微球涂层多孔钛对MG63细胞功能的影响。结果表明：明胶缓释微球涂层多孔钛无细胞毒性;当IGF-1、TGF-β1明胶缓释微球的载药浓度分别在0.1~10 ng/mg和0.25~2.5 ng/mg范围内时,与MG63细胞共培养,IGF-1和TGF-β1的载药浓度与细胞的增殖和分化呈正相关;当微球的载药浓度IGF-1为10 ng/mg,TGF-β1为2.5 ng/mg时,MG63细胞具有最优的增殖和分化;IGF-1和TGF-β1联合应用对MG63细胞的黏附、增殖与分化作用明显优于单一应用。

关 键 词：porous titanium gelatin microsphere insulin-like growth factor-1 transforming growth factor-β1 MG63 cell 

分 类 号：TQ460.1[化学工程—制药化工] Q2-33[生物学—细胞生物学]