复方861对糖尿病大鼠血、尿ngal的影响  

Effect of compound chinese herbal medicine 861 on NGAL in serum and urine in diabetic rats

作　　者：刘凤华[1] 张咪[1] 刘奇[1] 陈海平[1] 史振伟[1] 

机构地区：[1]北京煤炭总医院肾内科,北京100028

出　　处：《国际泌尿系统杂志》2015年第6期911-915,共5页International Journal of Urology and Nephrology

摘　　要：目的 探讨糖尿病肾病大鼠血、尿中性粒细胞明胶酶相关脂笼蛋白（neutrophil gelatinase associated lipocalin,ngal）的水平及复方861对其的影响.方法 采用一次性腹腔注射链脲佐菌素溶液制备糖尿病大鼠模型,随机分为正常对照组（N）、糖尿病组（D）、糖尿病中药治疗组（T）[中药复方861合剂1.5g·（kg·d）-1灌胃],于实验第2、4、8、12周处死,应用酶联免疫吸附法检测血清ngal（sngal）、尿ngal浓度（ungal）.结果 糖尿病组较正常组2周起血、尿ngal明显升高（P＜0.01）;治疗组较糖尿病组2周起尿ngal降低（P＜0.01）,4周血ngal明显降低（P＜0.01）.结论 血、尿ngal可作为糖尿病大鼠肾损伤的早期标记物.复方861可降低糖尿病大鼠血、尿ngal浓度而起到肾保护作用。Objectives To study the neutrophil gelatinase associated lipocalin （NGAL）in the serum and urine of diabetic rats and the efficacy of compound chinese？ herbal medicine 861 （ccm） on NGAL.Methods Diabetic rats were induced by intraperitoneal injection of streptozotocin （STZ）,and were randomly divided into three groups：normal control group （n =24）,d iabete group （n =24）,ccm [1.5 g · （kg · d）-1] treatment group （n =24）.The concentration of NGAL in serum（sngal） and urine（ungal） was measured by euzymelinked immunosorbent assay at the 2nd,4th,8th and 12th week of experiment.Results NGAL in serum,urine was increased significantly at the 2nd week in diabetic group;In the ccm group sngal and ungal were decreased at the 4th and 2th week respectively compared with the diabetes group.Conclusions NGAL in the serum,urine increases in the early time in diabetic rats and on the increase over time.Ccm can decrease ngal in serum,urine and alleviates the renal tubular ultramicrostructural injury to protect the kidney.

关 键 词：糖尿病 大鼠 蛋白质类 

分 类 号：R587.1[医药卫生—内分泌]