锰超氧化物歧化酶基因rs4880位点多态性与2型糖尿病发生及认知功能的相关性研究  

Relationship Between MnSOD-rs4880 Polymorphism and the Development of Type 2 Diabetes as Well as Cognitive Function

作　　者：甄艳凤[1] 刘兴宇[2] 房辉[1] 徐刚[3] 杨岳[1] 孙雪玲[1] 张谷月[1] 张丹丹[1] 许静[1] 周蕾[1] 

机构地区：[1]唐山工人医院内分泌二科,河北省唐山市063000 [2]唐山工人医院神经外科,河北省唐山市063000 [3]唐山工人医院烧伤科,河北省唐山市063000

出　　处：《中国全科医学》2015年第32期3939-3943,共5页Chinese General Practice

基　　金：河北省自然科学基金资助项目(H2015105083)

摘　　要：目的探讨锰超氧化物歧化酶(Mn SOD)基因rs4880位点多态性与2型糖尿病(T2DM)发生及认知功能的相关性。方法选取2008年3月—2010年3月唐山工人医院住院治疗的T2DM患者450例为病例组,同期于北京某社区招募无T2DM者512例为对照组。检测受试者空腹血糖(FPG)及三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)临床表型。采用重复性成套神经心理状态测验量表(RBANS)评价受试者认知功能。采用限制性片段长度多态性PCR技术检测Mn SOD基因rs4880位点多态性。结果Mn SOD基因rs4880位点基因型为TT、CT和CC。两组rs4880位点基因型及等位基因频率分布比较,差异均无统计学意义(P>0.05)。两组女性受试者rs4880位点基因型分布比较,差异有统计学意义(P<0.05);其中,CT基因型受试者发生T2DM的风险是TT基因型的2.188倍〔95%CI(1.413,3.388)〕。两组女性受试者rs4880位点等位基因频率分布比较,差异有统计学意义(P<0.05);其中,C等位基因发生T2DM的风险是T等位基因的1.926倍〔95%CI(1.310,2.830)〕。两组男性受试者rs4880位点基因型及等位基因频率分布比较,差异均无统计学意义(P>0.05)。病例组rs4880位点多态性与TC、LDL-C及胰岛素抵抗指数(HOMA-IR)相关(P<0.05),而未发现与FPG、TG、HDL-C相关(P>0.05)。病例组rs4880位点多态性与RBANS延迟记忆相关(P<0.05),而未发现与即刻记忆、视觉广度、言语功能、注意及总分相关(P>0.05)。结论 Mn SOD基因rs4880位点CT、C是女性T2DM发病的危险基因型和等位基因,T2DM患者RBANS延迟记忆评分与Mn SOD基因rs4880位点多态性有关。Objective To investigate the relationship between Manganese superoxide dismutase（ Mn SOD）- rs4880 polymorphism and the development of type 2 diabetes（ T2DM） as well as cognitive function. Methods We enrolled 450 T2 DM patients who were hospitalized in Tangshan Gongren Hospital from March 2008 to March 2010 as the case group,and we enrolled another 512 persons without T2 DM that were recruited from a community in Beijing in the same period as the control group. We collected the clinical phenotype data including FPG, TG, TC, HDL- C and LDL- C. The Repeatable Battery for the Assessment of Neuropsychological Status（ RBANS） was applied to assess the cognitive function of the subjects,and RFLP- PCR was used to detect Mn SOD- rs4880 polymorphism. Results Mn SOD- rs4880 has three genotypes： TT,CT and CC. There was no significant difference both in distribution of genotype and allele frequency between the two groups（ P 〉0. 05）. Significant difference was found in genotype distribution of this site in female subjects between the two groups（ P 〈0. 05）; subjects with CT genotype had 2. 188 times higher risk to develop T2 DM than TT genotype carriers 〔95% CI（ 1. 413,3. 388） 〕. Significant difference was also found in allele frequency of rs4880 site in female subjects between the two groups（ P 〈0. 05）; subjects who carried C allele had 1. 926 times higher risk to develop T2 DM than T allele carriers 〔95% CI（ 1. 310,2. 830） 〕. There was no significant difference both in the distribution of genotype and allele frequency of rs4880 site in male subjects between the two groups（ P 〉0. 05）. Association was found between rs4880 site polymorphism and TC,LDL- C and HOMA- IR（ P 〈0. 05）,while no association was found between rs4880 site polymorphism and FPG,TG and HDL- C（ P 〉0. 05）. The rs4880 site polymorphism was found associated with RBANS delayed memory（ P 〈0. 05）,while no association was found between Mn SOD- rs4880 polymorphism and immediate memory, visuospatial

关 键 词：糖尿病 2型 锰超氧化物歧化酶 rs4880位点 多态性 单核苷酸 重复性成套神经心理状态测验量表 认知障碍 

分 类 号：R587.1[医药卫生—内分泌]