不同频率糖皮质激素大鼠骨质疏松造模对软组织和免疫系统的影响  

Effects of different frequencies of glucocorticoids on the soft tissues and the immune system in a model of osteoporosis

作　　者：虎松艳[1] 汪源[2] 黄欣俊 唐璐[2] 李敏[2] 李青南[1,2] 陆幸妍[1,2] 

机构地区：[1]广东食品药品职业学院,广州市510520 [2]广东药学院生命科学与生物制药学院,广州市510006

出　　处：《实用医学杂志》2015年第22期3649-3653,共5页The Journal of Practical Medicine

基　　金：2010广东省实验动物重点实验室开放课题

摘　　要：目的:观察使用不同频率糖皮质激素(glucocorticoids,GCs)在骨质疏松造模过程中对大鼠的软组织形态学变化的影响,进而研究不同频率GCs的使用对大鼠免疫功能的影响。方法:32只3月龄SPF级SD雌性大鼠按体重随机分为4组:(1)正常对照组(C组);(2)低频率组(L组);(3)中频率组(M组);(4)高频率组(H组)。C组肌肉注射0.9%生理盐水;其他3组肌肉注射地塞米松(dexamethasone,Dex)1 mg/(kg·次),每周L组2次、M组4次、H组6次。每组在给药30 d后处死,取大鼠的软组织进行组织病理学观察及称量免疫器官重量。结果:(1)与C组相比,L、M、H组SD大鼠体重和免疫器官指数明显下降。(2)与C组相比,L、M、H组心、肺和肾的形态学变化为细胞水肿;肝脏组织脂肪变性;脾脏组织有轻度萎缩;胸腺严重萎缩。结论:GCs引起SD雌性大鼠组织病理学的变化,主要是引起胸腺严重退化,脾脏萎缩,且随着用药频率增加对机体免疫功能呈不同的抑制作用,频率越高,抑制作用越强。Objective To investigate different frequencies of glucocorticoids （GCs） on the tissue in a model of osteoporosis. Methods Thirtytwo three-month-old SD female rats were randomly divided into four groups： （1） the control group （group C）; （2） the low-frequency group （group L）; （3） the middle-frequency group （group M）; （4）the high-frequency group （H）. The rats in the group C were given intramuscular injection （im） of 0.9% saline. Im of dexamethasone （Dex） was 1 mg/（kg.time）. Rats were given two times im a week in the group C, four times im a week in the group M, and six times im a week in the group H. Each rat was sacrificed on thirty days post-administration. Results （1）The body weight of rats gradually increased in the Ctrl group, however, the body weight of rats declined gradually during the experiment in the group L, M and H. The size of immune organs （spleen and thymus） significantly decreased in rats of the group L, M and H. （2）Compared with the group C, cell edema was changed in the heart, renal and lung morphological fatty degeneration in liver, atrophy in spleen, atrophy in lymphoid nodules, and cell edema in kidney tubular were observed. Conclusion GCs cause serious degradation in the thymus and atrophy of the spleen. Administration has different inhibitory effect on immune function; the high frequency will lead to strong inhibition.

关 键 词：骨质疏松 糖皮质激素 软组织 免疫器官 

分 类 号：R580[医药卫生—内分泌] R-332[医药卫生—内科学]