基于1H NMR谱的给药赭石大鼠血清代谢组学研究  

A 1H NMR-Based Metabonomic Study on the Serum of Haematitum-Treated Rats

作　　者：魏莹[1,2] 章文军[1] 薛蓉[2] 李晓晶[2] 裴奉奎[2] 

机构地区：[1]河北工业大学化工学院,天津300130 [2]中国科学院长春应用化学研究所,吉林长春130022

出　　处：《波谱学杂志》2015年第4期628-636,共9页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金资助项目(20975097;21305134)

摘　　要：基于1H NMR的代谢组学方法对灌胃给药赭石的成年Wistar大鼠血清进行分析,运用主成分分析模式识别方法对所得数据进行处理,并对给药大鼠血清进行生化指标检测.研究结果表明,大鼠体内β-羟异丁酸、乙酸、丙酮、胆碱、甘油磷脂酰胆碱、葡萄糖、乳酸、低密度脂蛋白、极低密度脂蛋白和脂质等内源性代谢物浓度发生明显变化,可作为赭石的特征代谢物.2 g/kg和5 g/kg体重剂量赭石使大鼠机体产生大量活性氧化物(ROS),造成过氧化损伤,导致能量代谢和糖代谢紊乱,糖酵解反应增强,并且对肝功能造成了影响.Wistar rats were intragastrically administered with different doses（0, 2, 5 g/kg body weight） of haematitum. 1H NMR-based metabonomic techniques were used to analyze the metabolic profiles of the serum samples collected from the treated rats. Significant treatment-related changes were observed for the levels of β-hydroxybutyate, acetate, acetone, choline, glycerophosphorylcholine, glucose, lactate, low density lipoprotein, very low density lipoprotein and lipids. Such biochemical changes indicated that haematitum treatment at the dose of 2 or 5 g/kg body weight gave rise to reactive oxygen species（ROS） and caused peroxidation damage. It is concluded that haematitum treatment affects glucose, lipid and energy metabolism in rats.

关 键 词：核磁共振(NMR) 代谢组学 主成分分析 赭石 

分 类 号：O482.53[理学—固体物理]