MiR-106b对食管癌KYSE150细胞的生长及细胞上皮间质转化的影响  

MicroRNA-106b promotes proliferation of esophageal cancer cell and Epithelial-Mesenchymal Transition process

作　　者：戴芳[1] 刘涛[1] 郑树涛[1] 刘清[1] 王栋[1] 伊力亚尔.夏合丁 卢晓梅 

机构地区：[1]新疆医科大学第一附属医院临床医学研究院 [2]新疆维吾尔自治区食管癌研究所,乌鲁木齐830054

出　　处：《新疆医科大学学报》2015年第12期1466-1470,共5页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81160303;81260359;U1303321);新疆维吾尔自治区重大科技专项计划(201430123-1)

摘　　要：目的研究微小RNA106b(miR-106b)对食管癌KYSE150细胞增殖、迁移、侵袭及上皮间质转化(Epithelial-Mesenchymal Transition,EMT)的影响,为临床治疗食管癌提供新的理论依据。方法用miR-106b慢病毒转染KYSE150细胞,实验分为3组:control组(未转染miR-106b的KYSE150细胞)、miR-NC组(转染随机序列)和miR-106b组(转染miR-106b的KYSE150的细胞)。采用实时荧光定量聚合酶联反应(qRT-PCR)和免疫印迹(Western Blot)检测miR-106b和EMT的相关标记物E-钙黏蛋白(E-Cadherin)、神经型钙黏蛋白(N-Cadherin)的表达情况,四甲基偶氮唑盐微量酶反应比色法(MTT法)检测细胞的增殖能力,划痕实验检测细胞的迁移能力,Transwell方法检测细胞的侵袭能力。结果慢病毒稳定转染miR-106b后,食管癌细胞的增殖、侵袭和迁移能力均增强(P<0.05);qRT-PCR与Western blot检测显示miR-106b组E-Cadherin表达较miR-NC组、control组明显降低(P<0.05);N-Cadherin表达明显升高(P<0.05)。结论 miR-106b能够促进食管癌细胞的增殖、迁移和侵袭,同时能促进上皮间质的转化。Objective To investigate the effects of miR-106 b in the esophageal cancer cell proliferation,migration,invasion. Methods Based on KYSE150 cell transfected with miR-106 b,the expression of miR-106 b and EMT-associated markers were detected by quantitative RT-PCR（ qRT-PCR）. EMT-associated proteins were measured by western blot and cell proliferation was detected by using MTT assay. In addition,cell migration and invasion ability were evaluated by wound healing assay and transwell assay. Results The results showed that the cell proliferation,migration and invasion was significantly increased in esophageal cancer KYSE150（ P〈0. 05） after transfected with miR-106 b. Furthermore,expression of E-Cadherin was significantly lower in the group of transfected with miR-106 b than the control group（ P〈0. 05）. Meanwhile,expression of N-Cadherin was higher（ P〈0. 05）. Conclusion The present data revealed that miR-106 b could promote the cell proliferation and has potential in effect on EMT process in esophageal cancer.

关 键 词：miR-106b 细胞增殖 细胞迁移 细胞侵袭 上皮间质转化 

分 类 号：R34[医药卫生—基础医学] R655.4