冠心丹参方不同组分对大鼠急性心肌缺血模型的保护作用  

Protective Effects of Guanxindanshen Formula Different Components on Acute Myocardial Is chemia in Rats

作　　者：窦立雯 孙蓉[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250355

出　　处：《中国药物警戒》2015年第11期641-644,共4页Chinese Journal of Pharmacovigilance

基　　金：山东省自主创新和成果转化项目(2014ZZCX02104);国家科技部十一五支撑计划重点项目(2008BAI51B02);国家重大新药创制专项课题(2009ZX09502-015)

摘　　要：目的观察冠心丹参方不同有效成分群对冠脉左前降支造成的大鼠急性心肌缺血模型的保护作用。方法采用冠脉结扎建立大鼠心肌缺血模型,观察各组对大鼠冠脉结扎所致的心肌梗塞范围、心律失常室速(VT)和室颤(VF)发生率、血清SOD,MDA,LDH酶活性及心肌组织HE染色及组织病理学检查。结果冠心丹参方及其有效成分群各组均能明显对抗冠脉左前降支造成大鼠心肌梗塞范围,降低心律失常室速(VT)和室颤(VF)发生率,升高血清SOD,降低MDA、LDH的含量,保护心肌细胞。结论冠心丹参方及其有效成分群组均能够减轻冠脉左前降支造成大鼠心肌缺血性损伤,可能与其调节血清SOD、MDA、LDH,减轻缺血后的氧化应激反应,从而发挥抗心肌缺血的作用有关,对大鼠急性心肌缺血有一定的保护作用。Objective To compare the protective effects of Guanxindanshen formula（GDF） different components with effectiveness by ligating coronary left descending artery with acute myocardial ischemia in rats. Methods The rat model of acute myocardial ischemia was established by ligating coronary left descending artery into sublingual veirL The incidence rates of VT and VF in rats after ligating coronary left descending artery were observed. Myocardial infarction size （MIS） of model rats was calculated and pathological examination of myocardial tissue was performed. Meanwhile, the levels of lactate dehyd rogenase （LDH）, superoxide dismutase （SOD）, malondialdehyde （MDA） in serum were determined by assay. Results Compared with model group, the GDF group significantly reduced infarct size, decreased the levels of LDH and MDA in serum, and increased the activities of SOD. In addition, MIS was reduced and myocardial cells were protected. The GDF group was the best to protect myocardial ischemia. Conclusion GDF different components with effectiveness can significantly inhibit acute myocardial ischemia in rats caused by ligating coronary left descending artery.

关 键 词：冠心丹参方 冠脉左前降支 急性心肌缺血 保护作用 

分 类 号：R965[医药卫生—药理学] R289[医药卫生—药学]