乙氧氟草醚对泥鳅肝细胞的毒性研究  

Toxic Effects of Oxyfluorfen on Liver of Misgurnus anguillicaudatus

作　　者：夏晓华[1] 夏晓培 董慧[1] 卢辰华 徐培芳[1] 杜启艳[1] 常重杰[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《湖北农业科学》2015年第20期5092-5095,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31200923);河南师范大学青年骨干教师科研项目(5101049470610);河南师范大学引进人才科研项目(01046500109)

摘　　要：以泥鳅(Misgurnus anguillicaudatus)为试验对象,通过急性毒性试验、单细胞凝胶电泳技术(彗星试验)、酶活测定来检测除草剂乙氧氟草醚对泥鳅肝细胞的毒性效应,从而研究了乙氧氟草醚对水生生物的毒性。结果表明,乙氧氟草醚对泥鳅的24、48、72、96 h的半数致死质量浓度(LC50)分别为36.67、32.87、28.38、27.40 mg/L,安全浓度为7.92 mg/L。彗星试验各试验组中,浓度越高,细胞的拖尾越严重,呈现明显的时间-剂量效应。低浓度乙氧氟草醚处理(8、12 mg/L)对泥鳅肝脏谷丙转氨酶(GPT)和谷草转氨酶(GOT)具有诱导作用,而长时间(6 d)的高浓度乙氧氟草醚处理(16、20 mg/L)则具有抑制作用,试验组与对照组存在显著或极显著差异。Loach as test object, by acute toxicity test, single cell gel electrophoresis（cometassay）, enzyme activity assay to detectd oxyfluorfen fluorine grass ethers of loach liver cell toxicity effect. The toxicity of oxyfluorfen to aquatic organisms were studied. The results showed that, half of oxyfluorfen on the 24,48,72,96 h lethal concentration（LC50） were 36.67,32.87,28.38,27.40 mg / L, safe concentration for 7.92 mg / L. The experimental group, the higher concentration, the cell smearing more serious, also presents the obvious time dose effect. The activities of GPT and GOT in liver were induced by the lower concentration treatments（8,12 mg / L）, but restrained by the longer time（6 d） and higher concentration treatments（16,20 mg / L）. Experimental groups were significant difference or very significant difference in comparison with the control group. The results showed that the oxyfluorfen has a strong toxicity to Misgurnus anguillicaudatus.

关 键 词：乙氧氟草醚 泥鳅(Misgurnus anguillicaudatus) 半致死质量浓度 单细胞凝胶电泳技术 GPT GOT 

分 类 号：S948[农业科学—水产养殖]