豫北地区嗜水气单胞菌灭活疫苗的制备及免疫效果的研究  

Preparation of a deactivation vaccine against Aeromonas hydrophila and research about its immue effect in the north of Henan

作　　者：关建义[1] 毛会丽[2] 贺文旭 杨利敏[2] 符运栋 张敬梅[2] 吴佳静[2] 宁黔冀[1] 

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]新乡医学院生命科学技术学院,新乡453003

出　　处：《中国免疫学杂志》2015年第11期1501-1504,共4页Chinese Journal of Immunology

基　　金：河南省基础与前言技术研究项目(142300410021);河南省科技厅科技攻关(132102310165);河南省教育厅科技攻关资助项目(2011A240001);2013年国家大学生创新课题(201310472007)

摘　　要：目的:选取毒力因子多、毒性强的嗜水气单胞菌株(Aeromonas hydrophil)制成灭活疫苗,研究疫苗的免疫保护效果。方法:采用甲醛灭活制备疫苗,对银鲫进行腹腔注射,设立免疫组和对照组,然后进行血清抗体效价检测、病理切片分析和攻毒保护试验。结果:银鲫在经注射免疫后,各免疫时间均有抗体产生,抗体效价在第6周检测时达到高峰,而对照组在整个试验过程中均没有检测到抗体;病理切片也表明,该疫苗能够对鲫鱼靶器官产生很好的保护作用;攻毒保护试验中,免疫组的免疫保护率达100%,且免疫保护期长达6个月以上。结论:嗜水气单胞菌灭活疫苗对银鲫有显著的免疫保护效应,可作为预防细菌性败血症感染的疫苗。Objective： Selected virulence factors more than and high virulent Aeromonas hydrophia strain made into inactivated vaccine,to study the immunization effect of inactivated vaccine. Methods： Crucian were vaccinated with formalin-killed vaccine via intraperitoneal injection. Controls were injected with the same volumes of saline. Then the antibody titres,histopathology and relative percent survival were analyzed from samples of both groups. Results： The antibody in the indirect agglutination reaction could be detected in vaccinated fish once a weeks after immunization and reached highest level 6 weeks after immunization. The histopathology analysis indicated that the vaccine had a good protective effect on crucian target organs. Vaccinated fish showed 100% relative percent survival and the immune period would be 6 month. Conclusion： The vaccine in this study has a significant protective effect on crucian and may be used as effective fish vaccines against bacterial septicemia.

关 键 词：嗜水气单胞菌(Aeromonas hydrophil) 疫苗 抗体效价 病理切片 免疫保护率 

分 类 号：R392[医药卫生—免疫学]