药物代谢细胞色素酶催化活性检测新技术进展  

Methdological innovations in enzyme activity measurement of xenobiotic-metabolizing cytochrome P450

作　　者：刘妍妍[1] 程雨晴 谭桢[1] 郭宾[1] 

机构地区：[1]湖南师范大学化学生物学及中药分析教育部重点实验室化学化工学院,湖南长沙410081

出　　处：《化学研究》2015年第6期551-560,共10页Chemical Research

基　　金：国家自然科学基金项目(21005027;81274178)

摘　　要：细胞色素P450单加氧酶(CYP)能高效地催化种类繁多、结构迥异有机化合物的氧化反应,特别是非活性碳氢分子的单插氧反应,被誉为自然界的万能催化剂和机体最重要的药物解毒酶.本文作者对传统的CYP酶活性检测技术与若干新型分析方法的进展情况进行简要评述,主要综述了近年来出现的非传统代谢酶活性快速检测技术,如通过监测代谢反应过程中的氧气、辅酶或中间活性产物的变化,特别是采用电化学驱动电子转移、纳米杂化CYP替代酶源材料等交叉学科新方法,为发展新型CYP活性分析技术、深入探讨CYP催化反应机理提供新的思路.Cytochrome P450monooxygenase（CYP）can efficiently catalyze oxidation reaction of a wide variety of organic compounds with different structures,and it is known as the universal catalyst of nature and the most important drug detoxification enzyme of the body.In this article we briefly review the new progresses of traditional methods and a number of new analytical techniques of cross-disciplinary emerging in recent years for measuring CYP activity,including monitoring changes of oxygen,cofactor or reactive metabolites during the metabolic process,electrochemically driven electron transfer,alternative CYP nanohybrid materials,which provide new ideas for developing new type of analytical technologies and deepening the understanding of the catalytic mechanism of CYP-mediated metabolic reactions.

关 键 词：细胞色素P450 药物代谢 酶活性分析 技术进展 

分 类 号：O652[理学—分析化学]