溶酶体膜蛋白Sidt2对血糖代谢的影响  

Effects of lysosomal membrane proteins Sidt2 on glucose metabolism

作　　者：王李卓[1,2] 章尧[1,2] 余翠[2,3] 熊钱颖 谭凤彪 蒋雪[2] 陆晓华[4] 夏礼斌 陆美琴[3] 陈月平[3] 张斌华[3] 凌烈峰[1] 高家林[2,3] 

机构地区：[1]皖南医学院生物化学教研室,安徽芜湖241002 [2]皖南医学院活性生物大分子研究安徽省重点实验室,安徽芜湖241002 [3]皖南医学院第一附属医院弋矶山医院内分泌科,安徽芜湖241001 [4]皖南医学院机能实验中心,安徽芜湖241002

出　　处：《皖南医学院学报》2015年第6期518-521,共4页Journal of Wannan Medical College

基　　金：国家自然科学基金项目(81200632);安徽省自然科学基金项目(1308085QH134);弋矶山医院引进人才基金项目(2011014)

摘　　要：目的:通过模式动物研究溶酶体膜蛋白Sidt2与糖代谢的关系。方法:利用Lox P-Flox-Lox P系统,通过基因重组及打靶技术获得Sidt2剔除小鼠模型。检测成年小鼠的空腹血糖及葡萄糖耐量,评估Sidt2基因剔除对小鼠糖代谢的影响。同时通过HE染色观察Sidt2-/-小鼠胰岛形态结构改变。结果:通过对Sidt2剔除模型小鼠的Sidt2 mRNA及蛋白水平鉴定,确定Sidt2基因剔除小鼠模型成功构建。对小鼠末梢血糖检测发现,Sidt2-/-成年小鼠表现出空腹血糖增高及糖耐量受损的糖尿病表现。对Sidt2-/-小鼠胰岛形态检测发现,其胰岛出现结构紊乱,胰岛空泡变性及部分细胞出现肥大等病理改变。结论:溶酶体膜蛋白Sidt2与糖代谢密切相关。Objective： To discover the relationship between lysosome membrane protein Sidt2 and glucose metabolism through model animal. Methods：Sidt2 knockout mouse models were obtained by using Lox P-Flox-Lox P system,which based on the technologies of genetic recombination and gene targeting.By Fasting glucose and glucose tolerance test,the effects of Sidt2 deficiency on glucose metabolism were observed in Sidt2-/- mice. Furtherly,we analyzed the morphology change in Sidt2-/- mice islets,by hematoxylineosin staining. Results： Sidt2 knockout mice were produced successfully and verified by the analyzing the mRNA and protein expression of Sidt2. By peripheral blood glucose detecting,adult Sidt2-/- mice showed increased fasting glucose and impaired glucose tolerance. The pathology changes including disorder structure itself,islet cavity change and hypertrophic islet cells were also shown in Sidt2-/- deficiency mice at 12 months age,whereas the cotemporary controls showed normal islets. Conclusion： Lysosomal membrane protein Sidt2 is closely related to the glucose metabolism.

关 键 词：溶酶体膜蛋白 Sidt2 糖代谢 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]