硒化低聚氨基多糖制备工艺优化及其抗氧化活性研究  

Preparation of Seleno-aminopolysaccharide and Study on its Antioxidant Activity

作　　者：郭向阳 王友明[2] 高阳[3] 闻正顺[1] 关丽萍[1] 曲有乐[1] 

机构地区：[1]浙江海洋学院食品与医药学院,浙江舟山316022 [2]浙江大学动物科学学院,浙江杭州310058 [3]浙江海洋学院水产学院,浙江舟山316022

出　　处：《浙江海洋学院学报（自然科学版）》2015年第3期216-221,共6页Journal of Zhejiang Ocean University(Natural Science Edition)

基　　金：国家自然科学基金项目(31301982);浙江海洋学院2012年科研计划项目(面上项目)(X12M05);浙江海洋学院科研启动经费资助项目;浙江海洋学院校级重大项目(X12ZD08);浙江省教育厅2013年度科研计划项目(Y201328199)

摘　　要：硒化低聚氨基多糖(LSA)是由免疫活性的硒和低聚氨基多糖(LA)化合而成的硒多糖。通过正交试验,探讨了氨基多糖硒化修饰的最佳工艺条件,当硒化剂添加量为1.2 g,反应温度70℃,p H=3,反应时间8 h;硒化低聚氨基多糖中硒含量为27.3 mg/g。当硒化低聚氨基多糖浓度为4 mg/m L时,对DPPH、羟基自由基、超氧阴离子清除率分别为84.8%、71.6%、51.8%,还原力为0.33,硒化低聚氨基多糖的抗氧化能力要强于低聚氨基多糖。Low molecular seleno-aminopolysaccharide（LSA） is organic selenium compounds consisted of inorganic selenium and low molecular aminopolysaccharide（LA）. The optimal selenium modification conditions of low molecular aminopolysaccharide was studied by orthogonal experiment. When the dosage of selenium agent was 1.2 g, the temperature was 70 ℃, p H=3, and reaction time was 8 h, the selenium content was 27.3mg/g. The scavenging rate of DPPH free radical, hydroxyl radicals and superoxide anion free radical was84.8%, 71.6% and 51.8% and the reduction ability was 0.33 when the LSA concentration was 4 mg/ m L. The antioxidant capability of LSA was stronger than that of LA.

关 键 词：低聚氨基多糖 硒化 抗氧化 

分 类 号：Q539[生物学—生物化学]