血小板衍生内皮细胞生长因子转染脂肪间充质干细胞促进移植脂肪血管化  

Platelet-derived endothelial cell growth factor transfection of adipose-derived mesenchymal stem cells promotes vascularization of fat grafts

作　　者：伞光 宋佳 

机构地区：[1]无锡坤如玛丽亚医院整形科,江苏省无锡市214001 [2]无锡同济医疗美容医院整形科,江苏省无锡市214021

出　　处：《中国组织工程研究》2015年第41期6600-6605,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:血小板衍生内皮细胞生长因子在脂肪移植中可促进血运重建。目的:探索血小板衍生内皮细胞生长因子和脂肪间充质干细胞的双重促进脂肪移植成活率的作用。方法:1从新西兰大白兔腹股沟皮下脂肪中获取脂肪间充质干细胞,将第3代脂肪间充质干细胞分为实验组、对照组和空白组。实验组以Lenti-PD-ECGF-EGFP转染脂肪间充质干细胞,对照组以Lenti-EGFP转染脂肪间充质干细胞,空白组脂肪间充质干细胞未转染任何病毒。2取已被Lenti-PD-ECGF转染的脂肪间充质干细胞与DMEM完全培养基混合,然后与脂肪组织混合,作为A组;同样的方法用未转染任何病毒液的脂肪间充质干细胞制备B组,单独应用DMEM完全培养基而没有任何细胞成分的为C组。3组移植物注射于大白兔背部左上(A组)、左下(B组)、右上(C组)部皮下组织中。结果与结论:1实验组脂肪间充质干细胞中血小板衍生内皮细胞生长因子mR NA与蛋白的表达水平高于对照组和空白组,且细胞增殖速度也比对照组和空白组快。2A组移植物质量及毛细血管数量大于B组和C组。提示血小板衍生内皮细胞生长因子基因转染脂肪间充质干细胞后不仅可以持续的表达该蛋白,同时也能促进脂肪间充质干细胞的增殖。BACKGROUND： Platelet-derived endothelial cell growth factor（PD-ECGF） can promote revascularization in fat transplantation. OBJECTIVE： To explore the dual effects of PD-ECGF and adipose-derived mesenchymal stem cells on the survival rate of fat grafts. METHODS：（1） Adipose-derived mesenchymal stem cells were isolated from the inguinal subcutaneous fat of New Zealand white rabbits, and then cultured. Passage 3 adipose-derived mesenchymal stem cells were divided into experimental group（Lenti-PD-ECGF-EGFP transfected adipose-derived mesenchymal stem cells）, control group（Lenti-EGFP transfected adipose-derived mesenchymal stem cells） and blank group（adipose-derived mesenchymal stem cells with no transfection）.（2） Lenti-PD-ECGF-EGFP transfected adipose-derived mesenchymal stem cells were cultured in DMEM complete medium, and then mixed with fat tissues as group A; adipose-derived mesenchymal stem cells with no transfection were cultured in DMEM complete medium and then mixed with fat tissues as group B; DMEM complete medium with no cells served as group C. Then, the grafts in groups A, B, C were respectively injected subcutaneously into the upper left, lower left and upper right parts of the rabbits＇ black. RESULTS AND CONCLUSION：（1） In the experimental group, PD-ECGF m RNA and protein expressions were significantly higher than those in the control and blank groups（P〈0.05）, and cell proliferation was also the fastest.（2） Graft weight and the number of capillaries were greater in group A than groups B and C. These findingsindicate that PD-ECGF transfection of adipose-derived mesenchymal stem cells not only can continuously express the PD-ECGF protein, but also can promote the proliferation of adipose-derived mesenchymal stem cells.

关 键 词：转染 慢病毒感染 毛细血管 间质干细胞移植 组织工程 干细胞 脂肪干细胞 血小板衍生内皮细胞生长因子 脂肪间充质干细胞 脂肪移植 血管化 慢病毒 基因转染 血运重建 

分 类 号：R394.2[医药卫生—医学遗传学]