骨吸收抑制剂降钙素对骨关节炎大鼠软骨组织形态及蛋白多糖的影响  

Calcitonin effect on cartilage morphology and proteoglycan expression in rats with osteoarthritis

作　　者：陶冶 赵新友 房光 

机构地区：[1]济宁市兖州区人民医院骨科,山东省济宁市272000

出　　处：《中国组织工程研究》2015年第42期6735-6739,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:近年来有报道指出降钙素在临床上治疗骨关节炎有较好的临床疗效,但关于其对骨关节炎的作用机制却少有报道。目的:观察降钙素对实验性骨关节炎模型大鼠软骨组织形态及蛋白多糖的影响。方法:将30只SD大鼠随机均分为3组,模型组与给药组均切断右肢前交叉韧带制作骨关节炎模型,假手术组仅打开关节腔,不做韧带切断,给药组于造模后成功后第2天开始皮下注射降钙素15 IU/(kg·d),模型组与假手术组均皮下注射等量生理盐水,连续给药6周。造模后10周,观察各组大鼠胫骨关节面,X射线检查股骨远端和内外侧踝软骨下骨骨密度,苏木精-伊红染色观察骨组织形态,甲胺苯蓝染色观察软骨组织中蛋白多糖含量。结果与结论:假手术组大鼠胫骨关节面光滑,有光泽;模型组大鼠胫骨关节面无光泽,关节软骨暗红色,有较大溃疡面形成;给药组大鼠软骨面无光泽,局部溃疡,表面粗糙不平。与假手术组比较,模型组内外侧踝软骨下骨骨密度、骨体积、骨小梁数目升高(P<0.05),骨小梁分离度及蛋白多糖含量降低(P<0.05);给药组内外侧踝软骨下骨骨密度、骨体积、骨小梁数目低于模型组(P<0.05),骨小梁分离度及蛋白多糖含量高于模型组(P<0.05)。表明降钙素对骨关节炎大鼠软骨有较好的保护作用,并能促进骨组织分泌蛋白多糖。BACKGROUND：In recent years, calcitonin has been reported to have better clinical efficacy in the treatment of osteoarthritis, but its mechanism of action for osteoarthritis is rarely reported. OBJECTIVE：To investigate the effect of calcitonin on the cartilage morphology and proteoglycan expression in rats with osteoarthritis. METHODS：Thirty Sprague Dawley rats were randomly divided into three groups： model group, treatment group, and sham group. Anterior cruciate ligament transaction of the right limbs was implemented in the model and treatment groups, and only the joint cavity was exposed in the sham group. At 2 days after modeling, the treatment group received a daily subcutaneous injection of salmon calcitonin, 15 IU/（kg？d）, and the model group and sham group were administered with normal saline at the same dose. The injection lasted for 6 weeks. At 10 weeks after modeling, the articular surface of the tibia of rats in each group was generaly observed; bone mineral density of the distal femoral bone and subchondral bone of the lateral and medial ankle were detected using X-ray test; bone morphology and proteoglycan secretion were measured by hematoxylin-eosin staining and toluidine blue staining, respectively. RESULTS AND CONCLUSION：The tibial articular surface was smooth and glossy in the sham group, but oxblood in the model group with large-area ulcers; the treatment group showed rough and local ulceration. Compared with the sham group, the bone mineral density of the subchondral bone of medial and lateral ankle were increased significantly in the model group （P ＆lt; 0.05）, but trabecular separation and proteoglycan content were decreased （P ＆lt; 0.05）. Compared with the model group, the bone mineral density of the subchondral bone of＆amp;nbsp;medial and lateral ankle were decreased significantly in the treatment group （P ＆lt; 0.05）, while the trabecular separation and proteoglycan content were increased （P ＆lt; 0.05）. These findings indicate that calcitonin has bett

关 键 词：组织构建 软骨组织工程 降钙素 骨关节炎模型 骨关节炎 骨组织形态 蛋白多糖 

分 类 号：R318[医药卫生—生物医学工程]