不同种药物干预骨质疏松模型大鼠股骨压缩力学的变化  

Femoral compression variation in osteoporosis rats after intervention with different drugs

作　　者：齐兵[1] 戴维群[1] 尤建宇 李晓丽[1] 

机构地区：[1]吉林大学中日联谊医院药学部,吉林省长春市130031 [2]吉林大学中日联谊医院骨科,吉林省长春市130031

出　　处：《中国组织工程研究》2015年第42期6770-6775,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:力学性能指标是评价药物治疗骨质疏松动物模型效果的重要方法,以压缩力学性能指标评价多种药物治疗老龄雌性骨质疏松模型动物的效果评价鲜有报道。目的:对大鼠股骨进行压缩力学性能实验,确定不同药物治疗骨质疏松的效果。方法:Wistar雌性大鼠36只随机分为6组:正常组对照组、模型组,丹杞颗粒组,阿法D3组,结合型雌激素组,依普拉芬组,每组6只。除正常组对照组外以去卵巢法复制老龄骨质疏松动物模型,分别以丹杞颗粒、依普拉芬、结合型雌激素(倍美力片)、阿法D3进行治疗,饲养15周后以腹主动脉放血法处死大鼠,取大鼠左、右侧股骨在电子万能实验机上以5 mm/min实验速度进行压缩实验。结果与结论:骨质疏松模型组大鼠股骨最大载荷、最大应力、最大位移、最大应变、弹性模量显著低于正常对照组、丹杞颗粒、依普拉芬、结合型雌激素组(P<0.05);阿法D3组各项力学性能指标与模型组差异无显著性意义(P>0.05);丹杞颗粒治疗组上述指标与正常对照组大鼠差异无显著性意义(P>0.05)。结果说明,模型组大鼠由于骨质疏松导致股骨压缩力学特性发生改变。依普拉芬、结合型雌激素组均能提高大鼠股骨压缩力学特性,丹杞颗粒效果最好。BACKGROUND：The mechanical index is an important method for the evaluation of the therapeutic efficiency of drug treatment for osteoporosis animal models. OBJECTIVE： To explore the effects of various drug treatments on osteoporosis through a mechanical performance test about the femoral compression of rats. METHODS： Thirty-six Wistar female rats were randomized into six groups： normal control group, model group, Dan Qiparticles group, alpha-D3 group, premarin group, ipriflavone group, with six rats in each group. Osteoporosis models were made in al groups except for the normal control group, and after modeling, the rats in different groups were treated withDan Qi particles, alpha-D3 group, premarin and ipriflavone, respectively. After 15 weeks, the rats were kiled by abdominal aortic bloodletting to take out the left and right femurs that were placed on a universal testing machine to perform a compressive test at a speed of 5 mm/min. RESULTS AND CONCLUSION：The maximum load, maximum stress, maximum displacement, maximum strain, and elastic modulus were significantly lower in the model group than the other four groups （P ＆lt; 0.05）. There was no difference in different mechanical parameters between alpha-D3 group and model group as wel as between Dan Qi particles group and normal control group （P＆gt; 0.05）. These findings indicate that osteoporosis leads to the variation of compression mechanical properties of the femur. There are good compression mechanical properties of the femur after treatment with premarin and ipriflavone, andDan Qi particles has the best effect.

关 键 词：组织构建 骨组织工程 老龄 骨质疏松 动物模型 药物治疗 压缩 力学特性 

分 类 号：R318[医药卫生—生物医学工程]