星形胶质细胞纤维生长和突触形成的方向性和特异性  

Directional and specific characteristics of astrocyte fiber growth and synapse formation

作　　者：曹敬丽[1] 李奇[1] 王雅杰[1] 

机构地区：[1]首都医科大学附属北京天坛医院,临床医学研究实验室,北京市100050

出　　处：《中国组织工程研究》2015年第42期6787-6792,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81303268,81572474);北京市自然科学基金(7132094,7152098,7144209)~~

摘　　要：背景:星形胶质细胞除了被动地起代谢和结构支持的作用外,还能够以更复杂的方式与神经元相互作用,主动地参与中枢神经系统的信息处理。目的:通过观察星形胶质细胞纤维生长和突触形成的方向性和特异性,探索星形胶质细胞在与神经元相互作用中的可能机制。方法:取新生24 h内绿色荧光蛋白转基因小鼠,以原代细胞分离培养和爬片方法获得海马原代细胞,以胶质原纤维酸性蛋白荧光免疫组化方法特异性标记星形胶质细胞,在共聚焦显微镜下观察结果。结果与结论:星形胶质细胞纤维向距离较远的锥体细胞定向伸长,并且终止于锥体细胞的胞体,末端与锥体细胞的胞体形成突触样结构;而且并不是所有的突起都伸向临近的细胞,其突触样结构的形成也不全是在最临近的位点。表明星形胶质细胞纤维伸长和突触形成具有方向性和特异性,为进一步探索星形胶质细胞与锥体细胞之间的信号传导提供依据。BACKGROUND：Studies have shown that astrocytes participate not only in the passive metabolic and structural support, but also actively in the central nervous system information processing. OBJECTIVE：By observing the directionality and specificity of astrocyte fiber growth and synapse formation, to explore the possible mechanisms of astrocytes and neurons in the interaction. METHODS： Primary cels from the hippocampi of green fluorescent protein transgenic newborn mice were isolated and cultured, glial fibrilary acidic protein was used as specific marker for astrocytes in immunofluorescence staining, and then the results were observed and scanned by confocal microscope. RESULTS AND CONCLUSION：The elongation of astrocyte fibers was directional and specific, and terminated to the pyramidal cels nearby. The end of astrocyte fibers and pyramidal cels contributed to the synapse formation. However, not al the projections were toward the adjacent cels, and synapse-like structure was not only＆amp;nbsp;formed nearby. The results implicated that both astrocytes fiber elongation and synapse formation have the directionality and specificity, providing evidence for further studies on signal transduction between astrocytes and pyramidal cels.

关 键 词：组织构建 组织工程 绿色荧光蛋白 胶质原纤维酸性蛋白 星形胶质细胞 突触 血-脑屏障 锥体细胞 突起 海马 信号传导 原代细胞 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]