组织工程椎间盘纤维环:如何更接近于天然  

How to build a tissue-engineered intervertebral disc annulus fibrosus that is more close to the natural one?

作　　者：袁德超[1,2] 陈竹[1] 冯大雄 冯刚[1,2] 

机构地区：[1]川北医学院第二临床医学院组织工程与干细胞研究所.南充市中心医院,四川省南充市637000 [2]四川医科大学,四川省泸州市646000

出　　处：《中国组织工程研究》2015年第42期6832-6837,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81171472;81201407);四川省教育厅创新团队资助项目(13TD0030);四川教育厅重大培育项目(15CZ0021);南充市科技支撑计划项目(14A0021)~~

摘　　要：背景:组织工程椎间盘为椎间盘退行性变疾病的生物治疗提供了新的方法,组织工程纤维环是构建组织工程椎间盘的关键之一。目的:从纤维环结构特点、材料支架、种子细胞3方面,归纳总结组织工程纤维环的研究进展。方法:用计算机检索Pub Med、万方数据库,限定时间为2000至2015年,中英文检索词分别为"组织工程,椎间盘,纤维环,种子细胞,支架材料,构建"和"tissue engineering,intervertebral disc,annulus fibrosus,seed cell,scaffold,construction"。按纳入和排除标准对文献进行筛选,保留48篇文章进行综述。结果与结论:早期的组织工程纤维环的研究仅侧重于支架上的细胞黏附、细胞增殖和细胞外基质的分泌,目前的组织工程纤维环可以在细胞功能、组织结构和力学特点等方面比较接近于天然纤维环,在动物实验中取得了一定的成果。但是以目前的材料和技术,要构建完全类似于天然纤维环的组织工程纤维环仍具有一定的难度,支架材料、培养条件、种子细胞的获取等诸多方面仍需要进一步完善。目前的构建策略主要集中在单相的纤维环支架上,双相纤维环及完整椎间盘支架将是研究的趋势。干细胞的诱导分化技术为组织工程纤维环提供了广阔的种子细胞来源。BACKGROUND：Tissue-engineered intervertebral disc has provided a new biological therapeutic approach for intervertebral disc degeneration. Tissue-engineered annulus fibrosus is one key step of constructing a complete tissue-engineered intervertebral disc. OBJECTIVE：To sum up the research progress of tissue-engineered annulus fibrosus from the folowing aspects： structural features, scaffold materials, seed cels. METHODS：PubMed database and Wanfang database （2000-2015） were retrieved by the key words of “tissue engineering, intervertebral disc, annulus fibrosus, seed cel, scaffold, construction” in Chinese and in English, respectively. According to inclusion and exclusion criteria, 48 literatures were involved for summarization.＆amp;nbsp;RESULTS AND CONCLUSION： Previous studies about tissue-engineered annulus fibrosus only focused on cel adhesion, proliferation and extracelular matrix secretion on the scaffold. Currently, tissue-engineered annulus fibrosus exhibit similar features to the natural annulus fibrosus in the folowing aspects： cel function, tissue structure and mechanical features, and relevant animal experiments have achieved certain results in animal experiments. However, it is stil difficult to build a tissue-engineered annulus fibrosus entirely similar to the natural one, and we need to further improve scaffold materials, culture conditions, colection of seed cels. The current strategies of annulus fibrosus construction stil focus on single phase of scaffold, and the biphasic scaffold and complete intervertebral disc scaffold wil be the trend of the researches. Technology of induced differentiation of stem cels provides a broach source of seed cels for tissue-engineered annulus fibrosus.

关 键 词：组织构建 组织工程 组织工程纤维环 支架材料 种子细胞 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]