自固化磷酸钙人工骨Ⅱ型诱导成骨及修复腱骨界面损伤的生物力学分析  

Calcium phosphate cement II induces osteogenesis and repairs tendon-bone interface injury: a biomechanical analysis

作　　者：李小飞[1] 谢文瑾[1] 盛路新[1] 袁西[2] 

机构地区：[1]徐州医学院附属连云港市第一人民医院创伤骨科,江苏省连云港市222000 [2]徐州医学院,江苏省徐州市221002

出　　处：《中国组织工程研究》2015年第43期6889-6894,共6页Chinese Journal of Tissue Engineering Research

基　　金：江苏省卫生厅科研基金资助课题(Z6711)~~

摘　　要：背景:自固化磷酸钙人工骨Ⅱ型与重组人骨形态发生蛋白均有一定的成骨诱导作用,存在修复腱骨界面损伤的可能。目的:探讨自固化磷酸钙人工骨Ⅱ型的成骨诱导作用及修复腱骨界面损伤生物力学情况。方法:35只成年健康的新西兰白兔,随机选取5只,处死后取双侧肩关节腱骨界面标本作为正常对照。剩余30只构建腱骨界面损伤模型后,随机等分为实验组和模型组,模型组不填塞任何药物,实验组填塞自固化磷酸钙人工骨Ⅱ型进行修复。结果与结论:填塞自固化磷酸钙人工骨Ⅱ型后,兔损伤腱骨界面明显恢复,且随时间的延长,修复效果更佳,骨形态发生蛋白2表达水平也随之增加,损伤腱骨界面最大抗拉强度以及最大刚度明显增加。表明利用自固化磷酸钙人工骨Ⅱ型复合重组人骨形态发生蛋白对腱骨界面损伤进行修复具有良好的成骨诱导作用,可以促进损伤的修复。BACKGROUND： Both calcium phosphate cement II and recombinant human bone morphogenetic protein have certain osteoinductive effects,which have the possibility of repairing tendon-bone interface injury. OBJECTIVE： To investigate the osteoinductive effect of calcium phosphate cement II and its biomechanics analysis of repairing tendon-bone interface injury. METHODS： Five out of 35 adult healthy New Zealand white rabbits were randomly selected and their bilateral shoulder joint tendon-bone interface specimens were taken as normal control group after being sacrificed. The remaining 30 rabbits were used to make animal models of tendon-bone interface injury and then randomly divided into experimental and model groups. Rabbits in the model group had no treatment,and those in the experimental group were treated with calcium phosphate cement II. RESULTS AND CONCLUSION： After repair with calcium phosphate cement II,the injured tendon-bone interface of rabbits was obviously restored,and the repair effect became better with time. The expression level of bone morphogenetic protein 2 was also increased accordingly. The maximum tensile strength and the maximum stiffness of the injured tendon-bone interface were obviously increased. These results demonstrate that calcium phosphate cement II combined with recombinant human bone morphogenetic protein has good osteoinductive and repair effect in repair of tendon-bone interface injury.

关 键 词：磷酸钙类 腱 肩关节 假体和植入物 组织工程 生物材料 骨生物材料 肩袖 腱骨界面损伤 动物模型 自固化磷酸钙人工骨Ⅱ型 骨形态发生蛋白 生物力学 

分 类 号：R318[医药卫生—生物医学工程]