玄龙止咳颗粒中有效成分含量测定  

Determination of Effective Components in the XuanLong Cough Granule

作　　者：曹蕾[1] 杨红梅[1] 单丽芳[1] 宋英[2] 

机构地区：[1]成都中医药大学,四川成都610075 [2]成都中医药大学附属医院,四川成都610072

出　　处：《亚太传统医药》2015年第23期31-34,共4页Asia-Pacific Traditional Medicine

基　　金：四川省科技厅项目(CKY2014007)

摘　　要：目的:建立HPLC法测定玄龙止咳颗粒中盐酸麻黄碱,盐酸伪麻黄碱、哈巴俄苷含量的方法。方法:盐酸麻黄碱、盐酸麻黄碱的色谱条件为:Wondasil-C_(18)色谱柱(250mm×4.6mm,5μm),流动相甲醇-0.1%的磷酸(含0.1%的三乙胺)(4:96),流速1.0mL·min^(-1),温度30℃,进样量10μL,检测波长210nm;哈巴俄苷的色谱条件为:Kromasil 100-5-C_(18)色谱柱(250mm×4.6mm,5μm),流动相为0.03%的磷酸(A)-乙腈(B)梯度洗脱(0~10min,90%~97%A;10~20min,67%~90%A;20~25min,50%~67%A;25~30min,20%~50%A,30~35min,20%A;35~37min,20%~97%A),柱温35℃,检测波长280nm,流速1.0mL·min^(-1),进样量10μL。结果:盐酸麻黄碱、盐酸伪麻黄碱、哈巴俄苷线性范围分别为0.164 4~1.644 0μg(r=1.0),0.183 6~1.813 6μg(r=0.999 9),0.204 8~2.0480(r=0.999 9),平均加样回收率分别为100.56%(RSD=1.48%),100.58%(RSD=0.63%),99.83%(RSD=2.35%)。结论:建立的测定方法精密度高、重复性好,可用于玄龙止咳颗粒剂的质量控制,为其质量标准的建立奠定了基础。Objective：To establish an HPLC method for determining ephedrine hydrochloride and pseudoephedrine hydrochloride,harpagosides in XuanLong cough granule.Methods：HPLC conditions of ephedrine hydrochloride and pseudoephedrine hydrochloride were as follows：C_（18） column（250mm×4.6mm,5μm）,mobile phase was methanol-0.1%phosphate（including 0.1%of triethylamine）（4：96）,flow rate 1.0mL · min-1,column temperature 30 ℃,injection volume 10μL,detection wavelength 210nm;and conditions of harpagosides were：Kromasil 100-5-C_（18） column（250mm × 4.6mm,5μm）,the mobile phase was 0.03%phosphate（A）-acetonitrile（B） with gradient elution program（0-10min,97-90%A;10-20min,90-67%A;20-25min,67-50%A;25-30min,50-20%A,30-35min,20%A;35-37min,20-97%A）,column temperature 35℃,detection wavelength 280 nm,flow rate 1.0mL ·min-1,injection volume 10μL.Results：linear ranges of ephedrine hydrochloride and pseudoephedrine hydrochloride,harpagosides were 0.164 4-1.644μg（r=1.0）,0.183 6-1.813 6μg（r=0.999 9）,0.204 8-2.048μg（r=0.999 9）,the average recoveries of them were 100.56%（RSD=1.48%）,100.58（RSD=0.63%）,99.83（RSD=2.35%）.Conclusion：the established determination method was high precision and good reproducible,which can be used in the quality control of XuanLong cough granules,and laid the foundation to establish its quality standard.

关 键 词：玄龙止咳颗粒 盐酸麻黄碱 盐酸伪麻黄碱 哈巴俄苷 含量 

分 类 号：R284.2[医药卫生—中药学]