不同方法提取双黄二仙水提液中黄芪甲苷实验研究  

Study on different extraction methods of Shuanghuang Erxian aqueous extract of Astragaloside

作　　者：杨红梅[1] 曹蕾[1] 单丽芳[1] 宋英[2] 涂翔[2] 钟森[2] 

机构地区：[1]成都中医药大学,四川成都610075 [2]成都中医药大学附属医院,四川成都610072

出　　处：《亚太传统医药》2015年第23期35-37,共3页Asia-Pacific Traditional Medicine

基　　金：四川省中医药管理局青年基金(2014K108);成都市科技局科技惠民应用示范项目(2014-HM02-00014-SF);成都中医药大学附属医院科技发展基金(2013-D-YY-14)

摘　　要：目的:比较不同提取方法对双黄二仙颗粒水提液黄芪甲苷提取效果的影响,建立双黄二仙药液中黄芪甲苷含量测定方法。方法:分别采用超声法、大孔树脂法和水饱和正丁醇-氨试液萃取法提取药液中黄芪甲苷,使用HPLC-ELSD法测定其含量。结果:测定了3种提取方法下的黄芪甲苷的含量,建立了以水饱和正丁醇-氨试液的提取为该制剂提取黄芪甲苷的含量测方法。结论:该方法准确、灵敏、重现性好,专属性强,为该制剂质量标准的制定奠定了基础。Objective：To compare the effect of different extraction methods on the extraction of Radix astragalus of Shuanghuang Erxian aqueous extract.To establish a method for determination the astragaloside of Shuanghuang Erxian liquid.Methods：Using ultrasonic method,macroporous resin method and water saturated butanol-ammonia solution extraction liquid of Astragaloside,HPLCELSD method was used to determine its content.Results：Determination the content of Astragalus with three extraction methods,A method for the extract of Astragalus by water saturated butanol was established.Conclusion：The method is accurate,sensitive,reproducible and specific,which lays the foundation for the formulation of the quality standard of the preparation.

关 键 词：黄芪甲苷 提取工艺 含量测定 

分 类 号：R284.2[医药卫生—中药学]