谷胱甘肽S转移酶基因多态性与特发性男性不育精子DNA完整性相关性的研究  

Correlation between Glutathione S-transferase Polymorphisms and Sperm DNA Integrity in Male Patients with Idiopathic Infertile

作　　者：王欢[1] 孙发[2] 邢俊平[3] 丁上书[3] 孙超[2] 王新阳[3] 邹铁军[4] 唐开发[2,3] 

机构地区：[1]贵州医科大学电镜室,贵阳550004 [2]贵州医科大学附属医院泌尿外科,贵阳550004 [3]西安交通大学医学院第一附属医院泌尿外科,西安710061 [4]陕西省人民医院泌尿外科,西安710068

出　　处：《中国医科大学学报》2015年第12期1075-1078,共4页Journal of China Medical University

基　　金：国家自然科学基金(81300541);贵州省科学技术基金计划项目{黔科合J字[2012]2054号};贵阳医学院附属医院博士基金(C-2012-6)

摘　　要：目的探讨谷胱甘肽S转移酶基因M1、T1及P1(GSTM1、GSTT1及GSTP1)基因多态性与特发性男性不育症患者精子DNA断裂指数(DFI)的相关性。方法选择特发性少弱精子性男性不育症患者246例。采用聚合酶链反应及聚合酶链反应—限制性片段长度多态性方法,分别对GSTM1、GSTT1及GSTP1基因进行分型。采用吖啶橙染色标记流式细胞仪检测精子DNA完整性,并计算DFI。结果 GSTT1基因缺失型组特发性少弱精子性男性不育症患者精子DFI显著高于野生型组(P<0.01);而GSTM1基因缺失型组与GSTP1基因突变型组患者精子DFI与野生型组相比,差异无统计学意义(P>0.05)。结论 GSTT1基因缺失型与特发性男性不育症精子DFI呈正相关。Objective To investigate the correlation between glutathione S- transferase gene M1, T1 and P1 （GSTM1, GSTT1 and GSTP1）poly- morphism and sperm DNA fragmentation index （DFI） in male patients with idiopathic infertile. Methods The study included 246 male patients with idiopathic infertility. Polymerase chain reaction （PCR）and polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） were used to identify the genotype of GSTMI, GSTT1 and GSTPI, respectively. Sperm DFI was analyzed by flow cytometry with acridine orange staining. Results The sperm DFI was significantly higher in GSTT1 gene null type group than in GSTT1 gene wild type group （P 〈 0.01 ） ;and there were no significant differences in GSTM1 and GSTP1 gene mutation type groups when comparing with the wild type group（P〉 0.05 ）. Condusion GSTT1 gene deletion was positive associated with the sperm DFI in male idiopathic infertile patients.

关 键 词：男性不育症 谷胱甘肽S转移酶 基因多态性 DNA断裂指数 

分 类 号：R698.2[医药卫生—泌尿科学]