二氢丹参酮对人肺癌GLC-82细胞的抑制作用及其机制研究  

Inhibitory Effect and Its Mechanism of Dihydrotanshinone on Human Lung Cancer GLC-82 Cell

作　　者：孙蓓[1,2] 叶因涛[1] 王冬[1] 钱钧强[1] 娄建石[2] 

机构地区：[1]天津医科大学肿瘤医院/国家肿瘤临床医学研究中心/天津市"肿瘤防治"重点实验室,天津300060 [2]天津医科大学基础医学院,天津300070

出　　处：《中国药房》2015年第34期4775-4778,共4页China Pharmacy

基　　金：天津医科大学科学基金青年项目(No.2014KYQ06)

摘　　要：目的：研究二氢丹参酮（DTS）对人肺癌GLC-82细胞的抑制作用及其作用机制。方法：以质量浓度为0（空白对照）、5、10、20、40、80、100μg/ml的DTS作用细胞24、48 h后,采用MTT法测定细胞的增殖抑制率和半数抑制浓度（IC50）;流式细胞术检测17.85μg/ml DTS作用12、24、48 h后细胞的凋亡情况,计算凋亡率;Western blot法检测Bcl-2、Bax和半胱氨酸天冬氨酸蛋白激酶3（Caspase-3）蛋白的表达。结果：与空白对照组比较,DTS组细胞的增殖受到抑制,作用24、48 h后最大细胞抑制率分别为54.48%、64.95%,IC50分别为62.36、33.94μg/ml;DTS可诱导细胞的凋亡且与作用时间呈正相关,凋亡率为5.6%-29.6%;Western blot检测结果表明,DTS能下调细胞中Bcl-2蛋白的表达、上调Caspase-3蛋白的表达（P〈0.01或P〈0.05）。结论：DTS对人肺腺癌GLC-82细胞有较强的抑制作用,并诱导其凋亡,其机制可能与下调Bcl-2蛋白的表达、上调Caspase-3蛋白的表达有关。OBJECTIVE：To study the inhibitory effect of dihydrotanshinone（DTS）on human lung cancer GLC-82 cell and its mechanism. METHODS：After treated with 0（blank control）,5,10,20,40,80 and 100 μg/ml DTS for 24 and 48 h,MTT assay was used to measure the inhibition rates and IC50 of cells;cell apoptosis was detected by flow cytometry after treated with17.85 μg/ml DTS for 12,24 and 48 h to calculate apoptotic rate;Western blot was used to detect the protein expressions of Bcl-2,Bax and Caspase-3. RESULTS：Compared with blank control group,different concentrations of DTS inhibited the proliferation of cells;24 and 48 h maximal inhibition rate were 54.48% and 64.95%,respectively;IC50were 62.36 and 33.94 μg/ml. DTS could induce cell apoptosis in positive time dependent manner,and the range of inhibition rate was 5.6%-29.6%;Western blot showed DTS could down-regulate the expression of Bcl-2 protein and up-regulate the expression of Caspase-3 protein（P〈0.01 or P〈0.05）. CONCLUSIONS：DTS have significant inhibitory effect on GLC-82 cells and also induce cell apoptosis,by a possible mechanism of down-regulating the expression of Bcl-2 protein and up-regulating the expression of Caspase-3 protein.

关 键 词：人肺癌GLC-82细胞 二氢丹参酮 细胞凋亡 半胱氨酸天冬氨酸蛋白激酶3蛋白 

分 类 号：R285[医药卫生—中药学]