Axl和Mer受体调控小鼠红细胞分化  

Axl and Mer receptors regulate erythropoiesis in mice

作　　者：唐红梅 贾长虹 李巍伟 李明 

机构地区：[1]华北理工大学生命科学学院,河北唐山063000

出　　处：《基础医学与临床》2015年第12期1628-1632,共5页Basic and Clinical Medicine

基　　金：河北省自然科学基金(H2013209194)

摘　　要：目的研究Axl和Mer受体是否参与红细胞分化的调控。方法单基因(AXL-/-和MER-/-)和双基因(AXL-/-MER-/-)敲除小鼠,利用组织切片观察小鼠骨髓、肝脏和脾脏的组织结构;计数小鼠单根股骨内骨髓细胞的数量;用实时定量PCR检测与红细胞分化相关基因mRNA的表达。结果双基因敲除的小鼠单根股骨骨髓内总细胞、单个核细胞和成熟红细胞的数量分别下降了40%、34%和53%(P<0.01);骨髓细胞排列稀疏,血窦内、外成熟红细胞数量明显减少;虽然外周血中红细胞数量和血红蛋白的含量没有改变,但存在着明显的髓外造血,肝脏里出现了造血岛,脾脏中红髓明显地增生。而单基因敲除小鼠骨髓中红细胞数量以及组织结构未见改变。且双基因敲除小鼠中红系细胞内GATA-1和EpoR的mRNA表达分别降低了65%和75%(P<0.01)。结论 Axl和Mer受体通过GATA-1和EpoR来调节红细胞的分化,并且功能上存在重叠、过剩和代偿。Objective To study whether the Axl and Mer receptors are involved in the regulation of erythroid differ- entiation. Methods Single and double mutant mice for AXL and MER were used in the research. Histological sec- tions of bone marrows, livers and spleens, cellularity of bone marrow, hematologic parameters, and the expression of erythroid factors were analyzed to evaluate the function of Axl and Mer in erythropoiesis. Results Histological analysis showed that the sinusoids in wild-type bone marrows were filled with red blood cells, whereas those in the AXL-/- MER-/- bone marrows were largely empty and contained fewer red blood cells. The number of the total cells, the nuclearted cells and the red blood cells in an AXL-/- MER-/- femur were decreased. A compensatory extramedullary erythropoiesis occurred in AXL-/-MER-/- mice. The hematopoietic islands appeared in the liver, and the red pulp is expanded in the spleen. Mice lacking single AXL or MER exhibited normal erythropoiesis in steady-state conditions. The relative mRNA levels of EpoR and GATA- 1 were significantly down-regulated in AXL /- MER -/- erythroid cells. Conclusions Axl and Mer receptors may influence erythropoiesis through the reg- ulation of GATA-1 and EpoR expression in erythroid cells.

关 键 词：AXL MER 基因敲除 红细胞分化 

分 类 号：R346[医药卫生—基础医学]