糖尿病肾病患者血浆EMP与AECA的诊断价值  

Detection of plasma endothelial microparticles and anti-endothelial cell antibodies in patient with diabetic nephropathy

作　　者：刘建辉[1] 孙志平[2] 王琴[1] 王丽[1] 姚璐[1] 

机构地区：[1]河北省人民医院输血科,河北石家庄050051 [2]河北省胸科医院检验科,河北石家庄050041

出　　处：《热带医学杂志》2015年第11期1478-1480,共3页Journal of Tropical Medicine

摘　　要：目的检测糖尿病肾病患者血浆内皮微粒(EMP)、抗内皮细胞抗体(AECA)等相关指标的变化并探讨其临床诊断价值。方法应用流式细胞术检测血浆中EMP的水平,ELISA方法检测血浆AECA、血小板α-颗粒膜蛋白(GMP-140)和Von Willebrand因子(简称v WF)水平,并运用受试者工作特征(ROC)曲线分析其诊断价值。结果糖尿病肾病患者血浆EMP、AECA、GMP-140和v WF水平的检测水平明显高于正常对照组,且差异有统计学意义(P<0.01)。也较非糖尿病肾病组升高。EMP、AECA、GMP-140和v WF在ROC曲线下面积分别为0.940、0.993、0.623和0.680。结论糖尿病肾病患者血浆EMP、AECA、GMP-140和v WF水平明显增高,EMP和AECA的敏感性和特异性均较高,对诊断具有一定的意义。Objective To detect the level of plasma endothelial microparticles and anti-endothelial cell antibodies in patient with diabetic nephropathy and investigate the clinical diagnostic value. Methods The levels of endothelial microparticles,anti-endothelial cell antibodies, GMP-140 and v WF in plasma were measured by flow cytometry and ELISA. The receiver operating characteristic curve（ROC） was used to analyze the diagnostic value. Results The levels of endothelial microparticles, anti-endothelial cell antibodies and GMP-140 and v WF in plasma were higher than those of the control group（P〈0.01）.The areas under the ROC curve of EMP, AECA, GMP-140 and v WF were 0.940, 0.993,0.623 and 0.680, respectively. Conclusion The levels of endothelial microparticles, AECA, GMP-140 and v WF in plasma were obviously higher in patient with diabetic nephropathy.The sensitivity and specificity of EMP and AECAwere high, and could be used for the diagnosis.

关 键 词：糖尿病 肾病 内皮微粒 抗内皮细胞抗体 

分 类 号：R587.1[医药卫生—内分泌]