利用一级神经元重建完全性脊髓损伤大鼠后肢运动功能的实验研究  

RECONSTRUCTION OF LOWER EXTREMITY FUNCTION OF COMPLETE SPINAL CORD INJURY RATS BY FIRST NEURON CONNECTION

作　　者：王方永[1,2] 袁媛[1,2] 李建军[1,2] 

机构地区：[1]中国康复研究中心北京博爱医院脊柱脊髓外科,北京100068 [2]首都医科大学康复医学院

出　　处：《中国修复重建外科杂志》2015年第12期1528-1533,共6页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：北京市自然科学基金资助项目(7133253)~~

摘　　要：目的 探讨利用一级神经元重建完全性脊髓损伤大鼠后肢运动功能的效果。方法 取40只成年雌性SD大鼠,体质量300~350 g,制备L1椎体水平横断型完全性脊髓损伤模型;模型制备后2周,将大鼠随机分为对照组和实验组（n=20）;其中实验组行右后肢一级神经元直接重建手术,对照组除不缝合胫神经远端与股神经近端外,其余处理同实验组。术后观察大鼠一般情况,并于7、30、50、70 d对双侧后肢行BBB评分,观察后肢运动功能恢复情况;于70 d取材行HE染色、神经丝蛋白200（neurofilament 200,NF-200）免疫组织化学染色及辣根过氧化物酶（horseradish peroxidase,HRP）示踪标记染色观察脊髓重建区变化。结果 饲养期间6只大鼠死亡。两组右后肢运动功能无明显恢复,各时间点BBB评分均为0分;左后肢运动功能均有不同程度恢复,但各时间点两组BBB评分比较,差异均无统计学意义（P〉0.05）。实验组HE染色可见脊髓重建部位坐骨神经嵌入脊髓中,坐骨神经束膜显示清楚,连接部位脊髓无明显萎缩;NF-200免疫组织化学染色可见脊髓重建部位周围神经内神经元轴突呈阳性染色,且周围神经与脊髓形成联系。HRP逆行示踪标记染色示,实验组脊髓重建部位头端见HRP显色,对照组无着色。结论 一级神经元直接重建手术可使大鼠周围神经与中枢神经通过残留神经元轴突残端再生,重建相应的神经通路,但未见到周围神经支配靶肌肉运动功能的恢复。Objective To investigate the effects of the first neuron connection tor me reconstruction o, lowrl extremity function of complete spinal cord injury rats. Methods Forty adult female Sprague Dawley rats of 300-350 g in weight were selected to prepare the models of L1 transverse spinal cord injury. After 2 weeks of establishing model, the rats were randomly divided into control group （n=20） and experimental group （n=20）. In the experimental group, the right hind limb function was reconstructed directly by the first neuron; in the control group, the other treatments were the same to the experimental group except that the distal tibial nerve and the proximal femoral nerve were not sutured. The recovery of motor function of lower extremity was observed by the Basso-Beattie-Bresnahan （BBB） scoring system on bilateral hind limbs at 7, 30, 50, and 70 days after operation. The changes of the spinal cord were observed by HE staining, neurofilament 200 immunohistochemistry staining, and the technique of horseradish peroxidase （HRP） tracing. Results After establishing models, 6 rats died. The right hind limb had no obvious recovery of the motor function, with the BBB score of 0 in 2 groups; the left hind limb motor function was recovered in different degrees, and there was no significant difference in BBB score between 2 groups （P〉0.05）. In the experimental group, HE staining showed that the spinal cord was reconstructed with the sciatic nerve, which was embedded in the spinal cord, and the sciatic nerve membrane was clearly identified, and there was no obvious atrophy in the connecting part of the spinal cord. In the experimental group, the expression of nerve fiber was stained with immunohistochemistry, and the axons of the spinal cord were positively by stained and the peripheral nerve was connected with the spinal cord. HRP labelled synapses were detected by HRP retrograde tracing in the experimental group, group. Conclusion Direct reconstruction of the first neurons circuit by the growth of resi

关 键 词：脊髓损伤 一级神经元 皮质脊髓束 神经移植 大鼠 

分 类 号：R651.2[医药卫生—外科学]