2012-2013年福州市手足口病原学调查研究  

Etiological investigation of Hand Foot and Mouth Disease in Fuzhou from 2012 to 2013

作　　者：郑能雄[1] 陈霄璐 周美龄[2] 

机构地区：[1]福州市疾病预防控制中心,福建福州350004 [2]福建医科大学,福建福州350004

出　　处：《现代预防医学》2015年第23期4379-4383,共5页Modern Preventive Medicine

摘　　要：目的对2012—2013年福州市手足口病进行病原学调查研究并对其流行病学特征进行分析，旨在全面了解福州地区手足口病的肠道病毒。方法收集2012—2013年福州市区临床表现为手足口病的样本1031份，通过荧光定量RT-PCR、巢式RT—PER以及NCBI提供的BLAST序列比对确定肠道病毒型别。利用EVA6特异性引物鉴定2012—2013年福州市其他肠道病毒。对2012年EVA6手足口病例进行流行病学特征分析。结果1031份标本中878份显示EV阳性，检出率为85．16％（878／1031），其中EV71426例，阳性率为45．52％（426／878）；EVA1693例，阳性率为10．60％（93／878）；其他肠道病毒359例，阳性率为40．88％（359／878），其中CVA6为218例，阳性率为24．83％（218／878）。另还发现CVAl0和Ech03肠道病毒。流行病学特征分析发现0-5岁年龄组儿童为CVA6主要发病人群（构成比为94．04％），男女性别比为1．66：1；EVA6构成比情况在各月有较大差异，总体起伏明显，9月的CVA6病例构成比占62．50％，达到2012年最高峰。结论EV71、EVA16和CVA6是福州市手足口的主要肠道病原体，CVA6病毒已经超过CVA16成为第二大病原体，2012年CVA6主要发病人群为0—5岁儿童，9月份为发病高峰期。Objective The aim of this study was to investigate etiology and epidemiological characteristics of Hand Foot and Mouth Disease （HFMD） comprehensively, and to master and clarify the pathogenic spectrum and the genetic characteristics of HFMD in Fuzhou. Methods One thousand and thirty-one samples were collected from clinically diagnosed cases with HFMD. Real-time quantitative RT-PCR, nested RT-PCR and BLAST of VP1 sequences were carried out for nucleonic acid identification and typing. CVA6 specific primers were used to identify the other enteroviruses. Combined with epidemiological data, the distribution of CVA6 positive samples was analyzed. The epidemiological characteristics of CVA6 HFMD in Fuzhou were analyzed. Results Among the 1031 clinical samples, 878 （85.16%） cases were tested EV positive, including 426 cases of EVT1 positive with the positive rate of 45.52% （426/878）, 93 cases of CVA16 positive with the positive rate of 10.60% （93/878）, and 359 other enterovirus positive with the positive rate of 40.88% （359/878）. Among the other entemvirus positive samples, 218 cases of CVA6 positive with the positive rate of 24.83% （218/878） were found. CVAIO and Echo3 were identified. Epidemiological characteristics analysis found that 0-5 years children were the major morbidity crowd for CVA6 （94.04%）, with a gender ratio of 1.66： 1. And the situation of CVA6 had difference in time distribution. The constituent ratio was more than 62.50% in September, which reached the highest peak in 2012. Conclusion EV71, CVA 16 and CVA6 were the main intestinal pathogen of HFMF in Fuzhou. CVA6 had become the second largest pathogens over CVA16. CVA6 occurred mainly in children from 0-5 years old and entering the peak period of incidence in September of 2012.

关 键 词：手足口病 柯萨奇A6病毒 分子流行病学 

分 类 号：R195[医药卫生—卫生统计学]