重组人Ⅱ型TNF-α受体-抗体融合蛋白对胶原诱导性关节炎模型大鼠关节滑膜细胞NF-κB信号表达的影响  

Effects of recombinant human tumor necrosis factor-αreceptor Ⅱ∶Fc on NF-κB signal expressed in by synovial cells from CIA model rats

作　　者：姚血明[1] 马武开[1] 侯雷[1] 曹跃朋[1] 黄颖[1] 陆道敏[1] 宁乔怡 王敏 

机构地区：[1]贵阳中医学院第二附属医院,贵州贵阳550003 [2]贵州省中医院,贵州贵阳550001

出　　处：《中国医院药学杂志》2015年第22期2005-2007,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金(编号:81360580);贵州省社会公关项目[编号:黔科合SY字(2012)3123号];贵州省卫生厅科学技术基金项目(编号:yzwkj2012-1-05);国家中医药管理局重点建设专科项目;贵州省科技创新人才团队[编号:黔科合人才团队(20134033)];贵州省中医药民族重点学科项目(2012年)

摘　　要：目的:探讨重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白(rhTNFR∶Fc)对胶原诱导性关节炎(CIA)模型大鼠关节滑膜细胞NF-κB信号表达的影响。方法:将40只6周龄的雌性Wistar大鼠按照随机数表法随机分为空白组、模型组、醋酸泼尼松对照组和rhTNFR∶Fc治疗组,每组10只。制备CIA模型,以Western blotting法检测关节滑膜细胞NF-κB/P65、p-ⅠκBα表达的情况。结果:rhTNFR∶Fc治疗组和醋酸泼尼松对照组可下调CIA大鼠滑膜细胞NF-κB/P65、p-ⅠκBα的表达,与模型组比较均有显著性差异(P<0.05)。结论:rhTNFR∶Fc可通过降低CIA大鼠关节滑膜细胞NF-κB/P65、p-ⅠκBα蛋白表达以达到缓解炎症的作用。OBJECTIVE To discuss how recombinant human tumor necrosis factor-α receptor Ⅱ ：Fc （rhTNFR：Fc） influ- ences expression of NF-κB signal in synovial cells from CIA model rats and inflammatory factors. METHODS Forty six-week- old female Wistar rats were randomly divided into 4 groups including blank control group （n = 10）, model control group （n = 10）, treatment group with rhTNFR： Fc （n = 10） and prednisone acetate control group （n = 10）. Through establishing CIA model, detecting expression of NF-κB/P65 and p I κBα in synovial cells with Western blotting. RESULTS Compared with model control group, rhTNFR： Fc and prednisone acetate could visibly reduce expression of NF κB/P65 and p- I κBα in synovial cells from CIA model rats （P〈0. 05）. CONCLUSION rhTNFR： Fc can alleviate inflammation through remarkably reducing ex- pression of NF-κB/P65 and p-I κBα in synovial cells from CIA model rats.

关 键 词：rhTNFR FC 胶原诱导性关节炎 NF-κB信号 

分 类 号：R965[医药卫生—药理学]