“一测多评”法测定平卧菊三七中绿原酸类成分  

Quantitative analysis of multiple-components by a single marker of chlorogenic acids from Gynura procumbens(lour.)Merr

作　　者：李小军[1,2] 张妹砣 穆云妹 李婷婷[1] 杨艳羚[1] 曾紫璇 李玉桑[1] 唐和斌[1,2] 

机构地区：[1]中南民族大学药学院药理学研究室,湖北武汉430074 [2]中南民族大学,湖北省民族药物现代化工程技术研究中心,湖北武汉430074

出　　处：《中国医院药学杂志》2015年第22期2007-2011,共5页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:81403188);湖北省自然科学基金资助项目(编号:2013CFB451);湖北省民族药物现代化工程技术研究中心(中南民族大学)开放课题(编号:2015ZY002)

摘　　要：目的:分离鉴定平卧菊三七乙醇提取物所含绿原酸类成分,并用一测多评法建立其质量标准。方法:用制备型高效液相色谱法分离平卧菊三七中单体成分,利用液质联用、核磁共振法鉴定其结构。建立能准确测定其成分含量的HPLC方法,在此基础上,以绿原酸为对照,计算相对校正因子,建立"一测多评"法。结果:从平卧菊三七中分离纯化新绿原酸、绿原酸、异绿原酸A、异绿原酸B和异绿原酸C。相对校正因子的耐用性良好,一测多评法与外标法对比结果无显著差异。结论:异绿原酸A、B和C首次从本属植物中分离,所建立的含量测定方法可用于同时测定平卧菊三七提取物中5种绿原酸类成分。To study chemical constituents of G3rnura procunbens （lout.） Merr ethanol extract （GPEE） and to deter- mine chlorogenic acids by quantitative analysis of multi-components with a single marker （QAMS）. METHODS Chemical constituents of GPEE were studied by preparative HPLC. These compounds were identified by analysis of spectral data and chemical properties. Meanwhile, GPEE was analyzed by external standard method （ESM） and QAMS, respectively. Chlorogenic acid was used as an inter nal reference to calculate relative correction factors of four compounds. established is suitable for quality evaluation of GPEE.RESULTS Neochlorogenic acid, chlorogenic acid, isochlorogeaicacid A, B and C were identified in GPEE. Ruggedness of relative correction factors was good. And analytical results calculated by F.SM and QAMS showed no difference CONCLUSION Isochlorogenic acid A, B and C can be isolated from this genus for the first time. Quantitative method established is suitable for quality evalumion of GPEE.

关 键 词：平卧菊三七 一测多评 异绿原酸 绿原酸 

分 类 号：R927.2[医药卫生—药学]