盆炎灵颗粒的血清药物化学研究  

Study on serum pharmacochemistry of Penyanling granules

作　　者：肖作奇 文晓柯 潘涛[1,2] 欧阳波[1,2] 黄懿[1,2] 田霞[1,2] 邹威[1,2] 

机构地区：[1]湖南省妇幼保健院,湖南长沙410008 [2]湖南省中医药管理局一妇产科学中医药综合治疗重点研究室,湖南长沙410013

出　　处：《中国医院药学杂志》2015年第22期2012-2015,共4页Chinese Journal of Hospital Pharmacy

基　　金：湖南省中医药管理局科研项目(编号:201402);长沙市科技局科技项目(编号:K12ZD010-33);湖南省科技厅科技项目(编号:2013SK3212)

摘　　要：目的:通过对盆炎灵进行血清药物化学研究,初步阐明该复方的药效物质基础。方法:通过SD大鼠灌胃给予盆炎灵颗粒后,采用HPLC-DAD测定其含药血样、肝脏、肾脏、子宫的色谱图,通过与空白样品的HPLC指纹图谱对比分析,探讨盆炎灵的体内移行成分。结果:盆炎灵血清HPLC色谱图中可检出19个血中移行成分,其中11个成分被机体直接吸收入血,其余8个可能为代谢产物,5个色谱峰被确认为原儿茶醛、绿原酸、阿魏酸、甘草苷和丹酚酸B。含药肝脏组织中发现7个成分;含药肾脏中发现8个成分;含药子宫中发现2个成分。结论:含药组织的色谱峰明显多于空白组织,该方法为阐明盆炎灵的药效物质基础提供科学依据。OBjECTIVE To study serum pharmacochemistry of Penyanling granules and explore pharmacodynamic material basis through analyzing constituents absorbed in vivo. METHODS After SD rats were administrated Penyanling granules by gavage, chromatograms of serum, liver, kidney and uterus were measured by HPLC-DAD. Analysis and comparison were car- ried out for HPI.C fingerprints of blank tissues, medicated tissues and drugs to explore constituents absorbed after taking Pen yanling granules. RESULTS Nineteen transitional constituents to blood were were metabolite constituents. Five peaks were identified as protocatechuic al and salvianolic acid B. Seven constituents were found in liver, eight CONCLUSION Chromatographic peaks of medicated group are significantly provides some data on material basis study in wive） for Penyanling granules. detected, among which 11 were prototypes and 8 dehyde, chlorogenic acid, ferulic acid, liquiritin constituents in kidney, more than those of blank and two in uterus. group, and this study

关 键 词：盆炎灵 血清药物化学 物质基础 指纹图谱 移行成分 

分 类 号：R917[医药卫生—药物分析学]