天花粉蛋白裂解片段的抗原性研究  

Study on the antigenic determinants of trichosanthin fragments

作　　者：曹鹤年 马宝骊[1] 杨黎明 

机构地区：[1]上海第二医科大学免疫学教研室,上海200025 [2]上海市免疫研究所基础免疫一室

出　　处：《中国免疫学杂志》1991年第4期225-228,共4页Chinese Journal of Immunology

摘　　要：本文采用21株抗天花粉蛋白单抗对4个经溴化氰裂解的天花粉蛋白裂解片段进行抗原决定基分析。7株单抗特异结合天花粉蛋白裂解片段CBTf_1,2株单抗特异结合CBTf_2,2株单抗特异结合CBTf_4,7株单抗呈交叉反应,3株单抗与片段无结合反应。采用纯化的CBTf_1,CBTf_2对4株抗天花粉蛋白单抗的竞争结合实验显示:这两个片段对单抗的百分结合率可达到或接近50%,证明经溴化氰裂解的天花花粉蛋白片段仍保持原有的抗原性,这为天花粉蛋白结构和功能关系的研究提供重要资料。The antigenic determinants of Trichosanthin (TCS) were studied using cyanogen bromide cleavage of TCS and immunoblotting technique. 21 McAbs prepared against TCS could be divided into 5 groups according to the patterns of their reactivities with CNBr-fragments of TCS (CBTf1-4). (1) 7 antibodies bind to CBTf 1;(2) 2 antibodies bind to CBTf2;(3)2 antibodies bind to CBTf 4;(4)7 antibodies are cross-reactive with these fragments; (5) 3 antidodies bind to none of 4 fragments. Using two fragments CBTf1 and CBTf2 as competitors in a ELISA competition test, quantitative determination of antigenicity of TCS fragments by 4 McAbs to native TCS has shown that the maximal amount of bound antibody for CBTf1 with 2 McAbs reached 50% and for CBTf2 with another McAb nearly 50%. From these data, we concluded that TCS fragments have kept antigenic determinants as native TCS and it is very essential for us to study the structure-function relationship of TCS.

关 键 词：天花粉蛋白 裂解片段 抗原决定基 

分 类 号：R392.11[医药卫生—免疫学]