聚乙二醇琥珀酸酯1000与Soluplus对人参皂苷CK在Caco-2细胞模型上转运的影响  

Influence of TPGS 1000 and Soluplus on Membrane Transport of Ginsenoside CK in Caco-2 cell Monolayer Model

作　　者：金鑫[1,2] 谭晓斌[1] 严红梅[1] 于兆慧 贾晓斌[1] 张振海[1] 

机构地区：[1]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,南京210028 [2]宿迁市第一人民医院,江苏宿迁223800

出　　处：《中国药学杂志》2015年第22期1979-1982,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81403119)

摘　　要：目的考察聚乙二醇琥珀酸酯1000(TPGS 1000)与Soluplus对人参皂苷CK在Caco-2细胞模型上转运的影响。方法采用Caco-2细胞模型研究不同浓度的聚乙二醇琥珀酸酯1000与Soluplus对人参皂苷CK细胞转运行为的影响,以超高压液相色谱法(UPLC)测定细胞样品溶液中人参皂苷CK的浓度,计算表观渗透系数(Papp)与外排比率。结果当人参皂苷CK与聚乙二醇琥珀酸酯1000或Soluplus质量比例分别为1∶1、1∶3和1∶9时,人参皂苷CK吸收显著增加,外排显著降低,外排比率也随之显著下降(P<0.05);在相同比例条件下,聚乙二醇琥珀酸酯1000对人参皂苷CK的促吸收效果和抑制外排效果均优于Soluplus(P<0.05)。结论在Caco-2细胞模型上,聚乙二醇琥珀酸酯1000与Soluplus均可显著促进人参皂苷CK的吸收,有希望应用于CK制剂的开发中。OBJECTIVE To examine the effects of TPGS 1000 and Soluplus on the transport of ginsenoside CK in Caco-2 cell model. METHODS The effects of TPGS 1000 and Soluplus at different concentrations on ginsenoside CK were evaluated by using Caco-2 cell model. The concentration of ginsenoside CK in cell was examined by ultra high pressure liquid chromatography（ UPLC）method. The apparent permeability coefficient（ Papp） and the efflux ratio were calculated. RESULTS When the proportion of ginsenoside CK to TPGS 1000 or Soluplus was 1∶ 1,1∶ 3,and 1∶ 9,the absorption of ginsenoside CK significantly increased. The efflux and efflux ratio both decreased significantly（ P〈0. 05）. TPGS 1000 had more significant promotion effect on the transport of ginsenoside CK than the same dose of soluplus at the same ratio（ P〈0. 05）. CONCLUSION In Caco-2 cell model,both TPGS 1000 and Soluplus can significantly promote the absorption of ginsenoside CK.

关 键 词：人参皂苷CK CACO-2细胞模型 聚乙二醇琥珀酸酯1000 Soluplus 肠道吸收 

分 类 号：R944[医药卫生—药剂学] R965[医药卫生—药学]