核酸外切酶辅助的信号放大策略在生化分析中的应用进展  

Application Progress of Exonuclease-assisted Signal Amplification Strategies in Biochemical Analysis

作　　者：文立[1,2] 徐凤州[1,2] 何晓晓[1,2] 王柯敏[1,2] 何定庚[1,2] 卿太平[1,2] 邹振[1,2] 

机构地区：[1]化学生物传感与计量学国家重点实验室湖南大学生物学院 [2]湖南大学化学化工学院,生物纳米与分子工程湖南省重点实验室,长沙410082

出　　处：《分析化学》2015年第11期1620-1628,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21322509,21305035,21305038,21190044)资助~~

摘　　要：作为工具酶重要成员之一的核酸外切酶是一类无严格碱基序列依赖性的水解酶。近年来,通过利用核酸外切酶水解方式的差异性与纳米技术、酶切循环效应、核酸适配体技术、金属离子稳定的非沃森-克里克碱基配对系统、核酸荧光染料探针技术、电化学方法等相结合,发展了一系列核酸外切酶辅助的信号放大技术,对于提高生化分析检测方法与技术的灵敏度起到了非常关键的作用,已广泛应用于核酸、蛋白质、离子和小分子等物质的高灵敏检测。为了更好地理解和应用核酸外切酶辅助的信号放大策略,本文对核酸外切酶发展的信号放大策略在生化分析中的应用进展进行了综述。As an important member of tool enzymes,exonuclease is a kind of hydrolytic enzymes without strict base sequence dependent. In recent years,by taking advantage of different hydrolysis ways of exonuclease and nanotechnology,cycle effect of enzyme digestion,aptamer,non Watson-Crick base pairing system by metal ions,fluorescent nucleic acid probes,electrochemical methods etc.,a series of exonuclease-assisted signal amplification strategies have been developed,which played a very key role in improving the sensitivity of detection methods. Therefore,exonuclease has been widely used in high sensitive detection of nucleic acids,proteins,ions,small molecules and so on. To understand it better and apply it well in the future,the application progress of exonuclease-assisted signal amplification strategies in biochemical analysis has been summarized in this review.

关 键 词：分析检测 工具酶 信号放大 核酸外切酶 评述 

分 类 号：O652[理学—分析化学]