基于纳米通道表面增强拉曼散射光谱分离检测组氨酸对映体的研究  

Chiral Separation and Identification of D,L-Histidine Based on Nanochannels Membrane Coupling with Surface Enhanced Raman Scattering Spectroscopy

作　　者：钟桐生[1] 尹志芳[1] 柳悦[2] 黄杉生[1,2] 

机构地区：[1]湖南城市学院化学与环境工程学院,益阳413000 [2]上海师范大学生命与环境科学学院,上海200234

出　　处：《分析化学》2015年第11期1695-1700,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.21275100)资助~~

摘　　要：以Al2O3纳米通道膜为基体制备金纳米通道,以场发射扫描电镜、循环伏安、交流阻抗等方法对金纳米通道进行表征。采用EDC-NHS的交联反应,将壳聚糖自组装至金纳米通道孔壁上,形成表面具有手性位点选择性的功能化纳米通道膜,利用纳米通道优异的分离能力手性分离D-,L-组氨酸。考察了纳米通道孔径和溶液的p H值对分离效果的影响。采用银溶胶作为表面增强拉曼(SERS)测试的基底,增强对D-,L-组氨酸的SERS效应,提高检测该物质的选择性和灵敏度。分别在1000和1590 cm!1处测定L-组氨酸和D-组氨酸。在含200μL组氨酸、100μL银溶胶和100μL 80 mmol/L Na Cl溶液(p H=7.59)中,D-组氨酸和L-组氨酸可得到较好分离,分离度达到4.91。Gold nanochannels were prepared using Al2O3 nanotubules membrane as the carrier and modified with chitosan by a classical N-（ 3-dimethylaminopropyl）-N-ethyl carbodiimide（ EDC） / N-hydroxysuccinimide（ NHS） coupling reaction. The nanochannels were characterized by field emission scanning electron microscopy（ FESEM）,cyclic voltammetry and AC impedance method. The Au nanochannels modified with chitosan showed a chiral environment and can be used to separate histidine enantiomer. The effects of pore size and solution p H on the separation efficiency of histidine were investigated. To increase the detection sensitivity of D-,L-histidine,Ag nanoparticles were used to enhance the surface enhanced Raman scattering（ SERS）activity. The results showed that the chitosan-modified gold nanochannels can be used to separate chiral histidine based on this unique selective nanochannel membrane. L-Histidine and D-histidine were respectively detected by SERS at wavelengths of 1000 and 1590 cm-1. The results showed that L-histidine and D-histidine were separated well in the mixture containing 200 μL of histidine,100 μL of colloidal Ag and 100 μL of80 mmol / L Na Cl（ p H = 7. 59） with a separation efficiency of 4. 91.

关 键 词：金纳米通道 手性分离 表面增强拉曼光谱 组氨酸 

分 类 号：O657.37[理学—分析化学]