纸基-表面增强拉曼光谱法快速检测弱主药信号药品中的主药成分  

Rapid Detection of Active Pharmaceutical Ingredients in Weak Active Pharmaceutical Ingredient Signal Drugs by Paper Substrate-Surface Enhanced Raman Scattering Spectrometry

作　　者：李晓[1] 陈梦云[2] 王磊[2] 张倩倩[2] 方芳[2] 苗丽[2] 陆峰[1] 

机构地区：[1]福建中医药大学,福州350122 [2]第二军医大学,上海200433

出　　处：《分析化学》2015年第11期1735-1742,共8页Chinese Journal of Analytical Chemistry

基　　金：国家重大科学仪器设备开发专项资助项目(No.2012YQ180132)~~

摘　　要：建立了弱主药信号药品中主药成分的纸基-表面增强拉曼光谱(SERS)快速检测方法。采用浸泡法制备纳米银溶胶滤纸SERS基底(简称银胶纸),将待检样品滴加于银胶纸上进行SERS检测。通过考察银胶纸制备条件、银胶纸增强能力、SERS检测结果,建立用于弱主药信号药品中主药成分的快速检测方法。通过本方法获得弱主药信号药品中主药成分的SERS图谱,与其对应标准品图谱的相关系数大于0.9,较好地检测出弱主药信号药品中的主药成分,有效克服了常规拉曼光谱分析的不足之处。新型银胶纸制备简单、增强效果明显,与SERS法结合可简便、快速、准确地实现对弱主药信号药品中主药信号的检测,在弱主药信号药品快检中具有广阔的应用前景。A rapid detection method of active pharmaceutical ingredients（ API） in weak API signal drugs by surface enhanced Raman scattering（ SERS） technology combined with paper substrate was established in this work. By soaking the filter paper in silver nanoparticles solution（ Ag NPs） to synthesize Ag NPs-paper as the substrate,and then the sample solution was dropping on the substrate with SERS detection. On the basis of strengthen ability of Ag NPs-paper,result of SERS detection and optimal preparation conditions,the fast identification method of weak API signal drugs was established. In this case,the SERS spectra of weak API signal drugs and their standards SERS spectra were obtained,where the correlation coefficient of weak API signal drug SERS spectra and its standard was more than 0.9. The result showed that by this method,the low content API in weak API signal drugs could be well investigated,and the deficiencies of the normal Raman spectroscopy efficiently was also overcome. In conclusion,the synthesize method of Ag NPs-paper was simple,and the strengthen effect of this Ag NPs-paper on the intensity was obviously observed. Paper substrate-SERS method was simple,rapid and sensitive,and could be used to detect weak API signal drugs,presenting broad application prospects in the rapid detection of weak API signal drugs.

关 键 词：银胶纸 表面增强拉曼光谱 弱主药信号药品 相关系数 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.37[化学工程—制药化工]