干扰素α通过IFI16和P21影响人血管内皮细胞凋亡  

Interferon alpha induces apoptosis on human vascular endothelial cells via IFI16 and P21

作　　者：龙向淑[1] 吴强[1] 宋方[1] 黄晶[1] 田茂波[1] 肖燕[1] 

机构地区：[1]贵州省人民医院心血管内科,贵阳550002

出　　处：《重庆医学》2015年第34期4759-4761,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81260030)

摘　　要：目的观察干扰素α(IFN-α)对人血管内皮细胞(VECs)凋亡的影响并了解其部分机制。方法应用IFN-α和转染IFN诱导蛋白16基因(IFI16)的siRNA瞬时干预体外培养的VECs,以转染非特异性siRNA设为对照组,RT-PCR法检测IFI16及P21mRNA表达,Western blot检测IFI16及P21蛋白表达,流式细胞仪Annexin-V FITC/PI法检测细胞凋亡情况。结果与对照组比较,IFN-α组IFI16mRNA和蛋白表达上调(P<0.05),细胞凋亡增多(P<0.05),伴P21mRNA和蛋白表达增多(P<0.05);IFN-α干预后再转染IFI16siRNA,IFI16mRNA和蛋白表达下调(P<0.05),细胞凋亡减少(P<0.05),P21mRNA和蛋白表达减少(P<0.05)。结论 IFI16及P21参与IFN-α诱导VECs凋亡过程的调控。Objective To study the effect of interferon alpha （IFN-α） on apoptosis of human vascular endothelial ceils （VECs） and understand its partly mechanism, Methods Cuhured VECs were treated with IFN-α and interferon inducible protein16 gene（IFI16） siRNA was Set transfected in vitro instantaneously. Nonspecific siRNA transfection group was as control group. The expression of IFI16 and P21 mRNA Was determined by semiquantitative RT-PCR. IFI16 and P21 proteins were analyzed by Western blot. The cell apoptosis was analyzed by flow eytometry with Annexin-V FITC/PI method. Results Compared with control group, in IFN-a induction group,the expressions of IFI16 mRNA and protein were up-regulated（P〈0.05）, the cell apoptosis increased （P〈0.05） ,and the expressions of P21 mRNA and protein were up-regulated（P〈0.05） ;when transfection IFI16 siRNA after in-tervention with IFN-α, the expressions of IFI16 mRNA and protein were down-regulated （P〈0.05）, cell apoptosis decreased [ P〈 0.05） ,and the expressions of P21 mRNA and.protein were down-regulated（P〈：0.05）. Conclusion IFI16 and P21 participate in IFN-α regulation of cell apoptosis of VECs in human.

关 键 词：干扰素Α 干扰素诱导蛋白16 内皮 血管 凋亡 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]