小动物磁共振成像中不同麻醉方式的比较性研究  

A comparative study of different methods of anesthesia in small animal MRI

作　　者：郭宇[1] 张伟国[1] 方靖琴[1] 陈晓[1] 杜学松[1] 解添[1] 陈金华[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所放射科,重庆400042

出　　处：《重庆医学》2015年第34期4774-4776,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81271626);国家自然科学基金青年基金资助项目(81201139);重庆市自然科学基金资助项目(cstc2012jjB10028)

摘　　要：目的探讨在7.0T小动物磁共振研究中,注射麻醉与呼吸麻醉对实验动物和图像质量的影响及临床意义。方法选取80只200g左右的SD大鼠,随机分为两组,每组40只,其中,A组为注射麻醉组,B组为呼吸麻醉组。麻醉药物为异氟烷,预麻醉中麻醉剂量为0.5%,氧气为2lps,当动物深度麻醉后(双眼闭合,摇晃麻醉盒无反应时),倒计时2min,然后取出实验动物在检查床上进行固定,持续进行0.2%麻醉。固定所有动物时,均用医用纸胶带粘牢大鼠颈部,使大鼠头颅尽量与大鼠表面线圈接触,检查过程中所有动物均采用实时呼吸监控,进行常规头颅T1、T2、磁共振弥散加权成像(DWI)、灌注加权成像(PWI)成像。结果 A组实验动物在扫描过程中死亡4只,因呼吸逐渐上升,PWI检查前进行二次麻醉8只,B组实验动物均正常完成所有检查。B组实验动物较A组苏醒快;与A组比较,B组功能成像图像质量明显较好。结论实验中,小动物的状态可直接影响实验进程及实验结果的质量,而稳定、安全的麻醉是控制实验动物状态的有效方式。本研究中,呼吸麻醉可更好地控制大鼠的呼吸,并可保证动物的安全,从而提高实验的质量,为临床研究提供实验保障。Objective To explore the effect and clinical significance for experimental animals and image quality between in- jection of anesthesia and breathing anesthesia in 7.0 T small animal magnetic resonance（NMR） study. Methods A total of 80 SD rats of about 200 g were randomly divided into two groups （40 of each group）,in which A group for injection anesthesia group, group B for breathing anesthesia group, anesthetic was isoflurane, in the pre-anesthetic, we used 2 lps of oxygen with 0. 5% of isoflurane, when the animals were deeply anesthetized（closed eyes, shaking anesthesia box with no response）, continued 2 min, and then removed the experimental animals to the examinational bed,fixed them,continued used oxygen and isoflurane,but changed the isoflurane to 0.2%. When fixed all of the rats,cemented the neck of rats with medical tape and made the head of rats as far as possi- ble touch the surface coil of rats,we used real-time monitoring breathing for all animals during the process of examine,all examine just done T1,T2 ,DWI and PWI for regular head, Results In the group A, during the process of scanning, 4 rats were dead, and due to breathing gradually rise,8 rats had to do the secondary anesthesia before PWI. All rats of group B completed all the examines successfully. The rats in group B revived fast than those in group A. Compared with group A, the quality of function images were significantly better of group B. Conclusion In the experiment of small animal, the status of the experimental animals affects the quality of the images, the experiment process and the experimental results directly. The status of the experimental animals could be effectively control by stable and safe anesthesia way. In this study,breathing anesthesia could control rat respiratory condition very well, ensure the safety of the experimental animals and improve the quality of the experiment, this provid the experimental safeguard for clinical study.

关 键 词：磁共振成像 麻醉 动物实验 

分 类 号：R614[医药卫生—麻醉学]