IVF-ET治疗中短时受精对治疗结局的影响  

The Study of Short-time Fertilization on Treatment Outcome in IVF-ET

作　　者：马兰[1] 杨晓玲[1] 陈静思[1] 孟娇[1] 镡颖[1] 

机构地区：[1]昆明医科大学第二附属医院生殖医学科,昆明650101

出　　处：《昆明医科大学学报》2015年第11期108-111,共4页Journal of Kunming Medical University

基　　金：遗传资源与进化国家重点实验室开放课题(GREKF10-07)

摘　　要：目的比较分析IVF-ET周期中短时受精对受精、胚胎质量和妊娠结局的影响,明确短时受精在IVF-ET周期中的实施必要性.方法回顾性分析2013年10月至2014年1月就诊于昆明医科大学第二附属医院生殖医学科的392例IVF受精正常患者资料.患者平均年龄(32.39±4.71)岁,共获卵5 696枚,每个患者的卵随机分为短时受精组(A组)和过夜受精组(B组),A组卵子2 643枚,B组卵子3 053枚.分析2组正常受精率、成熟卵率、1PN率、3PN受精率、卵裂率、优质胚胎发育率、新鲜胚胎移植周期胚胎着床率和全胚冷冻周期行FET后妊娠率和胚胎着床率.结果分析2组之间成熟卵率、正常受精2PN率、1PN率、3PN率、卵裂率、优质胚胎率、新鲜胚胎移植周期妊娠率、胚胎着床率、全胚冷冻周期首次行FET后妊娠率和胚胎着床率,A组分别为88.84%、63.42%、2.88%、6.08%、98.56%、46.85%、44.57%、23.41%、49.06%和25.20%;B组分别为90.04%、65.56%、1.86%、5.87%、98.65%、42.49%、43.21%、23.23%、53.33%和33.33%.2组之间卵子成熟率、正常受精率、卵裂率、3PN率、新鲜胚胎移植周期妊娠率、胚胎着床率、全胚冷冻周期首次行FET后妊娠率和胚胎着床率差异无统计学意义(P>0.05),但短时受精组1PN率、优质胚胎率高于过夜受精组差异有统计学意义(P<0.05);新鲜周期和FET临床妊娠率和胚胎着床率差异有统计学意义(P<0.05).结论在IVF-ET的实施过程中,根据患者的病史,部分卵子选择短时受精-早期补救对于避免完全受精失败或者低受精率的发生具有一定的意义.短时受精增加异常受精率,妊娠率与过夜受精无差异.由于样本量的限制,此结果仍需要进一步的验证.Objective To analyze the influence of shorttime fertilization on the IVFET fertilization outcome and embryo quality and confirm the necessity of shorttime fertilization during IVFET.Methods We retrospectively analyzed the data selected from the 392 IVFET patients with normal fertility rate treated in the 2nd Affiliated Hospital of Kunming Medical University during Oct 2013 ∽Jan 2014, with the mean age was 32.39 ±4.71.The oocytes of every patient were randomly divided into two groups,groups of shorttime fertilization（group A） and overnight fertilization（group B）.2 643 oocytes of group A were fertilized after 40 hours of HCG injection and removed granulosa cells after about 6 hours to observe whether the second polar body had appeared,then 2PN was checked after 18∽20 hours of insemination.The oocytes from group B were inseminated after 40 hours of HCG injection and 2PN were checked after 18 ∽20 hours of insemination.Normal fertilization rate, maturation rate,1PN rate, 3PN rate, cleavage rate, highquality embryo rate, pregnancy rate of fresh embryo transfer,implantation rate, pregnancy rate of first FET cycle after cryopreservation of all embryos and its implantation rate were analyzed between the two groups.Results Maturation rate,normal fertilization rate,1PN rate,3PN rate,cleavage rate, good quality embryo rate, pregnancy rate of fresh embryo transfer, implantation rate, pregnancy rate of first FET cycle after cryopreservation of all embryos and it＇s implantation were respectively group A：88.84%、63.42%、2.88%、6.08%、98.56%、46.85%、44.57%、23.41%、49.06% and 25.20%;group B90.04% 、 65.56% 、 1.86% 、 5.87% 、 98.65% 、 42.49% 、 43.21% 、 23.23% 、 53.33% and 33.33%.No significant difference was observed between the two groups of maturation rate, normal fertilization rate, cleavage rate,3PN rate,pregnancy rate of fresh embryo transfer,implantation rate,pregnancy rate of first FET cycle after cryopreservation of all embryos and it＇s implantation.But 1PN rate and highquality e

关 键 词：IVF-ET 短时受精 受精率 胚胎质量 妊娠结局 

分 类 号：R321.33[医药卫生—人体解剖和组织胚胎学]