三个姓氏聚居村家系Y-STR单倍型分析（英文）  

Analysis of Y-STR Haplotypes by Pedigree in Three Surnameconcentrated Villages

作　　者：刘宏[1] 金国文 杨幸怡[1] 李越[1] 苏会芳[1] 刘超[1] 

机构地区：[1]广州市刑事科学技术研究所广东省法医遗传学重点实验室,广州510030 [2]佛山市南海区公安局,广东佛山528200

出　　处：《刑事技术》2015年第6期450-456,共7页Forensic Science and Technology

基　　金：supported by grants from Guangdong Key Laboratory of Forensic Genetics(2010A060801001);Guangdong Scientific and Technological Project(2013B021500010);Applied-innovation Project of Ministry of Public Security(2011YYCXGDST076)~~

摘　　要：目的利用Y-STR单倍型建库进行家系排查,对相对封闭的姓氏聚居区域发生的案件,或以姓氏亲缘为纽带的团伙性案件的侦破,具有其它技术不可替代的作用。近年来,国内已有相关报道与实际应用,但针对姓氏聚居区域Y-STR单倍型分布特点的基础研究缺乏,Y-STR家系数据库建设与家系比对排查也缺少理论支撑。本文通过对3个聚居姓氏家系样本的Y-STR单倍型检测分析,探讨如何更好地使用Y-STR单倍型做家系排查及数据库建设。方法以姓氏聚居的自然村为研究区域,以五代父系亲缘为一个家系单位,按区域内该姓氏家系总数l/5随机选取家系,采集该家系内全部男性个体样本,共获3个姓氏72个家系426份样本。其中,河北廊坊市某镇某村苏姓30个家系155份样本,杨姓20个家系92份样本;广西岑溪市某村林姓22个家系179份样本。应用Y-filer^(TM)试剂盒对上述样本进行Y-STR单倍型检测,并对获得的单倍型进行分析。结果检测的3个姓氏72个家系426个体的Y-filer系统,共获得46个单倍型,各单倍型所包含的个体数量在群体中分布不均衡,并且根据差异大小呈"集合"分布。其中,苏姓30个家系中共有10个单倍型,分为5个单倍型集合;杨姓20个家系中共有14个单倍型,分为两个集合;林姓22个家系中共有22个单倍型,形成4个集合。不同姓氏间未见相同的单倍型。如根据单倍型在同姓中的分布特点,将同源集合里包含最多家系数的代表性单倍型定义为主流单倍型,以主流单倍型作为该姓氏Y-STR的"标识物",则苏姓中的H1,杨姓H11、H12、H17和林姓H25、H36为主流单倍型。以主流单倍型作为比对对象,基因座容差范围设置为2,可覆盖122名苏姓、59名杨姓和130名林姓人员,取样群体占比分别为78.7%、64.1%、72.6%。72个家系的17个Y-STR基因座共观察到19次突变,其中苏姓30个家系共1 13次父子遗传,2个家系共发生3次突变,平均突变�Objective To investigate into the screening,pedigree identification and database construction of Y-STR＇s main haplotypes.Methods Haplotypes at 17 Y-STR gene loci of 426 males from 72 pedigrees having descended beyond five generations in three surname-concentrated villages were surveyed and analyzed with Y-filer TM kit,and the number of haplotypes was counted along with the coverage rate of main haplotypes.Results A total of 46 haplotypes were detected at17 Y-STR gene loci from the 72 pedigrees investigated,with each surname＇s pedigrees having about 1 or 2 main haplotype（s）which were originated to form the other mutated or varied ones.The population coverage rate was 62%~79%when the fault allowance for variance set at two gene loci.Conclusions Database of Y-STR＇s main haplotypes by surnamed-pedigree,once set up,could help to reduce the scope to find suspects,target at certain pedigree,though inability to directly identify the suspects from a same surname-concentrated community.

关 键 词：法医遗传学 Y-STR单倍型 Y-STR突变 父系排查 Y-STR数据库 

分 类 号：D919.6[医药卫生—法医学]