广西汉族与苗族人群30个InDels的遗传学调查  

Forensic Application of 30 InDel Loci in Guangxi Han and Miao Population

作　　者：赵蕾[1] 袁晚红 王雪倩[1] 孙启凡[1] 欧元[1] 孙敬[1] 魏以梁[1] 赵兴春[1] 李彩霞[1] 

机构地区：[1]公安部物证鉴定中心北京市现场物证检验工程技术研究中心,北京100038 [2]瑞金市公安局,江西瑞金342500

出　　处：《刑事技术》2015年第6期497-499,共3页Forensic Science and Technology

基　　金：中央级公益性科研院所基本科研业务费项目(2014JB010)

摘　　要：目的 采用Investigator？DIPplex试剂盒调查广西汉族、苗族中的群体遗传学数据,并评估其法医学应用价值。方法 对广西汉族、苗族的100名无关个体的30个In Dels位点进行复合扩增,统计分析各位点的频率数据、群体遗传学参数。结果 经Bonferroni校正,30个In Dels位点的分布均符合Hardy-Weinberg平衡;各位点在广西汉族、苗族人群中的累积个体识别率分别为0.999 999 999 98、0.999 999 999 97,累积非父排除率均大于0.96。30个In Dels在广西汉族中6个位点（D111、D118、D99、D114、D64、D39）He〈0.3,苗族中8个位点（D111、D118、D99、D122、D64、D81、D39、D84）He〈0.3。通过Genepop 4.2软件计算Fst值,除D84（0.080 7）外,其余位点均小于0.06。结论 该组30个In Dels位点在广西汉族、苗族人群中具有较高的多态性,可以作为现有STR检验体系的补充。Objective To study 30 insertion/deletion polymorphisms （InDels） of Guangxi Han and Miao population and evaluate their forensic applicability. Methods 30 InDels and amelogenin were determined with Investigator DIPplex in 50 unrelated individuals in the population of Han and Miao in Guangxi. Allele frequencies were measured along with other population genetics parameters. Results No significant deviation from Hardy-Weinberg equilibrium or linkage disequilibrium after Bonferroni＇s correction was observed in 30 InDels loci of Guangxi Han and Miao population. The total probability of discrimination power （TDP） was 0.99999999998 and 0.99999999997 for Guangxi Han and Miao population, respectively. The cumulative probability of exclusion （CPE） was greater than 0.96 for both populations. Out of the 30 InDels loci, six of He〈0.3 for Guangxi Han population localized on Dlll, Dl18, D99, Dl14, D64 and D39, and for Guangxi Miao population on the eight loci of D 111, D 118, D99, D 122, D64, D8 l, D39 and D84. The Fst values, calculated through Genepop 4.2 software, were less than 0.06 for all loci but D84 （0.0807）. Conclusions The 30 InDels are highly polymorphic in Guangxi Han and Miao population and can be used for individual identification, both supplementary and stand-alone.

关 键 词：法医遗传学 插入缺失多态性 个体识别 广西汉族 苗族人群 

分 类 号：Q347[生物学—遗传学]