低温胁迫下脱落酸对金钗石斛膜透性和内源激素的影响  

Effect of Exogenous Abscisic on Cell Membrane and Endogenous Hormone Contents in Leaves of Dendrobium nobile Under Low Temperature Stress

作　　者：陈明辉[1] 张志录[1] 佟伟霜[1] 杨风岭[1] 

机构地区：[1]平顶山学院低山丘陵区生态修复重点实验室,河南平顶山467000

出　　处：《北方园艺》2015年第23期74-78,共5页Northern Horticulture

基　　金：河南省科技厅科学攻关计划资助项目(122102310652);河南省环境科学重点学科资助项目(Pxy-zdxk-2013003);平顶山学院高层次人才科研启动经费资助项目(PXY-BSQD-2014007)

摘　　要：低温是限制金钗石斛生长发育和分布的主要环境因子之一。现以金钗石斛为试材,研究了低温胁迫下ABA处理对叶片细胞膜透性及内源激素含量的影响,以期为ABA在金钗石斛栽培中的应用提供参考依据。结果表明:低温胁迫下,金钗石斛叶片细胞膜受破坏,GA3、IAA、ZR含量下降;相对电导率、ABA、JA、SA含量升高。外施ABA能有效缓解低温胁迫对细胞膜的影响,降低GA3、IAA、ZR含量,提高ABA、SA含量,从而提高金钗石斛的抗寒性。试验表明低温条件下,外施ABA能降低金钗石斛细胞膜透性,调节不同激素水平,提高抗寒性。Low temperature is one of the main environmental factors which limit Dendrobium nobile growth,development and distribution.The effect of cell membrane permeability and the levels of endogenous hormones in leaves of D.nobile seeding were studied under cold stress and ABA treatment,in order to provide scientific evidence for the application of abscisic acid（ABA）in the production and cultivation of D.nobile.The results showed that low temperature treatment decreased the concentrations of GA3,IAA,ZR in leaves of D.nobile and injured the cell membrane,while the relative electric conductivity,the contents of ABA,JA,SA were increased under the low temperature stress.The exogenous ABA could reduce the membrane permeability and the contents of GA3,IAA,ZR in leaves,but the contents of ABA and SA were increased,reflecting the improvement of cold resistance of D.nobile seedling.The results clearly suggested that the foliar application of exogenous ABA could protect cell membrane permeability and adjust the different hormone levels against cold conditions.

关 键 词：低温胁迫 脱落酸(ABA) 金钗石斛(Dendrobium nobile Lindl) 细胞膜 内源激素 

分 类 号：S682.19[农业科学—观赏园艺]