凝固酶阴性葡萄球菌生物被膜形成及耐药性分析  

Biofilm formation and antimicrobial resistance of coagulase-negative staphylococcus

作　　者：胡洪华[1,2] 杨永长[2] 肖代雯[2] 姜伟[2] 周薇[2] 喻华[2] 传良敏[2] 刘华[2] 黄文芳[2] 

机构地区：[1]西南交通大学材料学院,四川成都610031 [2]四川省医学科学院四川省人民医院,四川成都610072

出　　处：《中国感染控制杂志》2015年第11期721-725,共5页Chinese Journal of Infection Control

基　　金：四川省卫生厅科研课题(140077;120072)

摘　　要：目的分析临床血培养报阳的凝固酶阴性葡萄球菌(CNS)生物被膜形成能力及耐药性特征。方法收集某院临床血培养报阳的CNS 126株,采用96孔聚苯乙烯培养板分析生物被膜形成能力,聚合酶链反应(PCR)法检测细菌耐药基因mecA,并分析菌株的耐药情况。结果 126株CNS中,87株(占69.04%)生物被膜阳性,105株(83.33%)携带mecA基因。CNS对青霉素、苯唑西林及红霉素的耐药率均>80%,未发现对利奈唑胺、奎奴普丁/达福普汀、万古霉素和替加环素耐药的菌株;耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)对环丙沙星、庆大霉素、左氧氟沙星、青霉素、利福平和复方磺胺甲口恶唑的耐药率均高于甲氧西林敏感凝固酶阴性葡萄球(MSCNS)(均P<0.05)。对苯唑西林敏感的CNS中,有2株mecA检测阳性。结论临床血液来源的CNS具有较高的生物被膜形成能力,绝大部分为MRCNS,且表现为多重耐药;存在耐药表型与基因型不一致的菌株。Objective To analyze the biofilm forming ability and antimicrobial resistance of coagulase-negative staphylococcus（CNS）isolated from blood culture.Methods 126 strains of CNS from blood culture in a hospital were collected,biofilm formation was analyzed with 96-well polystyrene plates,and polymerase chain reaction （PCR） was applied to detect mecA gene,antimicrobial resistance was analyzed.Results Among 126 CNS isolates,87 （69.04%）were positive for biofilm,105 （83.33%）carried mecA gene.The resistant rates of CNS to penicillin, oxacillin,and erythromycin were all〉 80%,none of the strains were resistant to linezolid,quinupristin/dalfopris-tin,vancomycin,and tigecycline;resistant rates of methicillin-resistant CNS （MRCNS）to ciprofloxacin,gentami-cin,levofloxacin,penicillin,rifampicin,and compound sulfamethoxazole were all higher than methicillin-susceptible CNS（MSCNS）（all P 〈0.05）.Of MSCNS,2 carried mecA gene.Conclusion CNS isolated from blood have strong biofilm forming ability,most are MRCNS and with multidrug resistance;there are strains with inconsistence of drug-resistant phenotype and genotype.

关 键 词：葡萄球菌 凝固酶阴性葡萄球菌 生物被膜 抗药性 微生物 病原菌 

分 类 号：R378.11[医药卫生—病原生物学]