Novozym 435催化酮洛芬酯化拆分研究  

Kinetic resoulution of ketoprofen using Novozym 435

作　　者：马燕[1] 杨震炯 丁雁[1] 黄和[1] 胡燚[1] 

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,江苏南京210009 [2]永康市质量监督检测中心,浙江永康321300

出　　处：《南京工业大学学报（自然科学版）》2015年第6期54-60,68,共7页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：国家杰出青年科学基金(21225626);国家高技术研究发展计划(863计划)(2011AA02A209);江苏高校优势学科建设工程

摘　　要：通过工艺优化确定Novozym 435催化酮洛芬酯化拆分的适宜条件为:酰基受体正丙醇与反应介质异辛烷体积比为1∶9、酮洛芬质量浓度20 mg/mL、反应温度30℃、转速200 r/min,24 h后反应的转化率和产物对映体过量值(eep)分别为580%和782%。研究表明:丁二酸酐和顺丁烯二酸酐修饰的Novozym 435对正丙醇的耐受性获得显著提升,正丙醇在反应体系中与异辛烷的体积比可分别达到3∶7和2∶8,同时底物酮洛芬浓度可分别提高到40和80 mg/mL。而且,添加剂的加入能进一步改善酶的催化性能,反应20 h后,丁二酸酐修饰的Novozym 435在以β环糊精为添加剂时,转化率及ee_p值为486%和812%;经顺丁烯二酸酐修饰的Novozym 435在以PEG为添加剂时的转化率和ee_p值为489%和801%。The optimal conditions of the esterification of ketoprofen by Novozym 435 were deterimined as follows：n-propanol as the acyl aceeptor, isooctane as the reaction medium and the ratio of 1：9, the concentration of ketoprofen of 20 mg/mL, temperature of 30℃, and rotate speed of 200 r/min. After reaction of 24 h, the conversion ratio and the eep value were 58.0% and 78.2%, respectively. Results showed that n-propanol tolerance of Novozym 435 modified by succinie anhydride and maleic anhydride could be improved, the volume ratios of n-propanol to isooctane increased to 3 ： 7 and 2 ： 8, respectively. Besides, the concentrations of ketoprofen reached up to 40 and 80 mg/mL. Moreover, the additives could further improve the catalytic properties of lipase.The conversion ratio and the eep value of Novozym 435 modified by suceinic anhydride were 48.6% and 81.2% using β-eyclodextrin as the additive; the conversion and eep value of Novozym 435 modified by maleic anhydride were 48.9% and 80. 1% when using PEG as the additive.

关 键 词：酮洛芬 Novozym 435 化学修饰 添加剂 

分 类 号：TQ033[化学工程]